Genetic Evidence Against Intramolecular Rejoining of the Donor DNA Molecule Following IS10 Transposition

Overview

Journal Genetics

Publisher Oxford University Press

Specialty Genetics

Date 1991 Aug 1

PMID 1655563

Citations 11

Authors

J Bender

J Kuo

N Kleckner

Affiliations

Soon will be listed here.

Abstract

Tn10 and IS10 transpose by a nonreplicative mechanism in which the transposon is excised from the donor molecule and integrated into a target DNA site, leaving behind a break at the original donor site. The fate of this broken donor DNA molecule is not known. We describe here two experiments that address this issue. One experiment demonstrates that a polar IS10 element gives rise to polarity-relief revertants at less than 1% the frequency of transposition of the same element in the same culture. In a second experiment, transpositions of an IS10 element from one site in the bacterial genome to another are selected and the resulting isolates examined for alterations at the donor site; none of 1088 such isolates exhibited a detectable change at the donor locus. These results are compatible with two possible fates of the transposon donor molecule: degradation ("donor suicide"), or restoration of the original information at the donor site by a recombinational repair mechanism analogous to double-strand break repair. These results argue against the possibility that the donor molecule gap is simply resealed by intramolecular rejoining.

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