Pseudorabies Virus GIII and Bovine Herpesvirus 1 GIII Share Complementary Functions

Overview

Journal J Virol

Publisher American Society for Microbiology

Date 1991 Oct 1

PMID 1654451

Citations 8

Authors

X P Liang

L A Babiuk

T J Zamb

Affiliations

Soon will be listed here.

Abstract

The gIII glycoproteins of bovine herpesvirus 1 (BHV-1) and of pseudorabies virus (PRV) are structurally homologous. Both proteins also play preeminent roles in mediating virus attachment to permissive cells. To directly compare the functional relation between these glycoproteins, we constructed a recombinant BHV-1 in which the BHV-1 gIII coding sequence was replaced by the PRV gene homolog. The resultant recombinant virus efficiently expressed PRV gIII and then incorporated it into its envelope. The levels of PRV gIII expression and incorporation were equivalent to those achieved by the wild-type virus for BHV-1 gIII. The recombinant virus was fully susceptible to neutralization by anti-PRV gIII neutralizing antibody. In addition, the virus attachment and penetration functions, as well as the virus replication efficiency, which were lost by deleting the BHV-1 gIII gene, were restored by expressing the PRV gIII homolog in its place. These results demonstrated that PRV gIII and BHV-1 gIII share complementary functions.

Citing Articles

BHV-1: new molecular approaches to control a common and widespread infection.

Turin L, Russo S, Poli G Mol Med. 1999; 5(5):261-84.

PMID: 10390543 PMC: 2230419.

Heparin-binding domain of bovid herpesvirus 1 glycoprotein gIII.

Okazaki K, Honda E, Kono Y Arch Virol. 1994; 134(3-4):413-9.

PMID: 8129625 DOI: 10.1007/BF01310578.

A peptide-model for the heparin-binding property of pseudorabies virus glycoprotein III.

Sawitzky D, Voigt A, HABERMEHL K Med Microbiol Immunol. 1993; 182(6):285-92.

PMID: 8121329 DOI: 10.1007/BF00191944.

Glycoprotein E of pseudorabies virus and homologous proteins in other alphaherpesvirinae.

Jacobs L Arch Virol. 1994; 137(3-4):209-28.

PMID: 7944945 DOI: 10.1007/BF01309470.

Cell surface proteoglycans are not essential for infection by pseudorabies virus.

Karger A, Saalmuller A, Tufaro F, Banfield B, Mettenleiter T J Virol. 1995; 69(6):3482-9.

PMID: 7745695 PMC: 189061. DOI: 10.1128/JVI.69.6.3482-3489.1995.

References

Liang X, Babiuk L, van Drunen Littel-van den Hurk S, Fitzpatrick D, Zamb T . Bovine herpesvirus 1 attachment to permissive cells is mediated by its major glycoproteins gI, gIII, and gIV. J Virol. 1991; 65(3):1124-32. PMC: 239878. DOI: 10.1128/JVI.65.3.1124-1132.1991. View

Wudunn D, Spear P . Initial interaction of herpes simplex virus with cells is binding to heparan sulfate. J Virol. 1989; 63(1):52-8. PMC: 247656. DOI: 10.1128/JVI.63.1.52-58.1989. View

Schreurs C, Mettenleiter T, Zuckermann F, Sugg N, Ben-Porat T . Glycoprotein gIII of pseudorabies virus is multifunctional. J Virol. 1988; 62(7):2251-7. PMC: 253365. DOI: 10.1128/JVI.62.7.2251-2257.1988. View

Cai W, Gu B, Person S . Role of glycoprotein B of herpes simplex virus type 1 in viral entry and cell fusion. J Virol. 1988; 62(8):2596-604. PMC: 253689. DOI: 10.1128/JVI.62.8.2596-2604.1988. View

Johnson D, Ligas M . Herpes simplex viruses lacking glycoprotein D are unable to inhibit virus penetration: quantitative evidence for virus-specific cell surface receptors. J Virol. 1988; 62(12):4605-12. PMC: 253572. DOI: 10.1128/JVI.62.12.4605-4612.1988. View

Robbins A, Watson R, Whealy M, Hays W, Enquist L . Characterization of a pseudorabies virus glycoprotein gene with homology to herpes simplex virus type 1 and type 2 glycoprotein C. J Virol. 1986; 58(2):339-47. PMC: 252918. DOI: 10.1128/JVI.58.2.339-347.1986. View

Fuller A, Spear P . Specificities of monoclonal and polyclonal antibodies that inhibit adsorption of herpes simplex virus to cells and lack of inhibition by potent neutralizing antibodies. J Virol. 1985; 55(2):475-82. PMC: 254956. DOI: 10.1128/JVI.55.2.475-482.1985. View

Hughes G, Babiuk L, van Drunen Littel-van den Hurk S . Functional and topographical analyses of epitopes on bovine herpesvirus type 1 glycoprotein IV. Arch Virol. 1988; 103(1-2):47-60. PMC: 7087229. DOI: 10.1007/BF01319808. View

van Drunen Littel-van den Hurk S, Babiuk L . Synthesis and processing of bovine herpesvirus 1 glycoproteins. J Virol. 1986; 59(2):401-10. PMC: 253090. DOI: 10.1128/JVI.59.2.401-410.1986. View

10.

Fitzpatrick D, Babiuk L, Zamb T . Nucleotide sequence of bovine herpesvirus type 1 glycoprotein gIII, a structural model for gIII as a new member of the immunoglobulin superfamily, and implications for the homologous glycoproteins of other herpesviruses. Virology. 1989; 173(1):46-57. DOI: 10.1016/0042-6822(89)90220-1. View

11.

Zuckermann F, Zsak L, Reilly L, Sugg N, Ben-Porat T . Early interactions of pseudorabies virus with host cells: functions of glycoprotein gIII. J Virol. 1989; 63(8):3323-9. PMC: 250905. DOI: 10.1128/JVI.63.8.3323-3329.1989. View

12.

Mettenleiter T . Glycoprotein gIII deletion mutants of pseudorabies virus are impaired in virus entry. Virology. 1989; 171(2):623-5. DOI: 10.1016/0042-6822(89)90635-1. View

13.

Johnson D, Wittels M, Spear P . Binding to cells of virosomes containing herpes simplex virus type 1 glycoproteins and evidence for fusion. J Virol. 1984; 52(1):238-47. PMC: 254511. DOI: 10.1128/JVI.52.1.238-247.1984. View

14.

Hampl H, Ben-Porat T, Ehrlicher L, HABERMEHL K, Kaplan A . Characterization of the envelope proteins of pseudorabies virus. J Virol. 1984; 52(2):583-90. PMC: 254561. DOI: 10.1128/JVI.52.2.583-590.1984. View

15.

Robbins A, Weis J, Enquist L, Watson R . Construction of E. coli expression plasmid libraries: localization of a pseudorabies virus glycoprotein gene. J Mol Appl Genet. 1984; 2(5):485-96. View

16.

Mettenleiter T, Zsak L, Zuckermann F, Sugg N, Kern H, Ben-Porat T . Interaction of glycoprotein gIII with a cellular heparinlike substance mediates adsorption of pseudorabies virus. J Virol. 1990; 64(1):278-86. PMC: 249100. DOI: 10.1128/JVI.64.1.278-286.1990. View

17.

Sawitzky D, Hampl H, HABERMEHL K . Comparison of heparin-sensitive attachment of pseudorabies virus (PRV) and herpes simplex virus type 1 and identification of heparin-binding PRV glycoproteins. J Gen Virol. 1990; 71 ( Pt 5):1221-5. DOI: 10.1099/0022-1317-71-5-1221. View

18.

Fitzpatrick D, Zamb T, Babiuk L . Expression of bovine herpesvirus type 1 glycoprotein gI in transfected bovine cells induces spontaneous cell fusion. J Gen Virol. 1990; 71 ( Pt 5):1215-9. DOI: 10.1099/0022-1317-71-5-1215. View

19.

Johnson D, Burke R, Gregory T . Soluble forms of herpes simplex virus glycoprotein D bind to a limited number of cell surface receptors and inhibit virus entry into cells. J Virol. 1990; 64(6):2569-76. PMC: 249433. DOI: 10.1128/JVI.64.6.2569-2576.1990. View

20.

Kuhn J, Kramer M, Willenbacher W, Wieland U, Lorentzen E, Braun R . Identification of herpes simplex virus type 1 glycoproteins interacting with the cell surface. J Virol. 1990; 64(6):2491-7. PMC: 249424. DOI: 10.1128/JVI.64.6.2491-2497.1990. View