Presence of Immunounreactive Albumin in the Urine of Diabetic Patients

Overview

Journal J Clin Lab Anal

Publisher Wiley

Specialties Biomedical Engineering
Biotechnology
Pathology

Date 2006 Mar 16

PMID 16538642

Citations 1

Authors

Aki Nakayama

Takashi Ida

Yoshiji Hatano

Kayoko Oohara

Hiroshi Yoshida

Toshiaki Baba

Hisashi Sakamoto

Kiyoko Shiba

Affiliations

Soon will be listed here.

Abstract

Recent studies have demonstrated that conventional immunochemical assays underestimate urinary albumin concentration because of the presence of immunounreactive albumin. It has been reported that intact urinary albumin in 24-hr diabetic urine samples could be detected as total concentration (immunoreactive+immunounreactive) by an HPLC method based on size exclusion chromatography. The aim of this study was to investigate urinary albumin concentration in diabetic spot urine samples by comparing the HPLC method with several other methods. The albumin concentrations on 80 diabetic spot urine specimens were measured by turbidimetric immunoassay (TIA), high performance liquid chromatography (HPLC), and a dipstick method. In addition, they were also analyzed by reducing sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS PAGE) and native polyacrylamide gel electrophoresis (Native PAGE). The albumin concentrations derived from diabetic spot urine samples measured by the HPLC method were higher than those of the other methods except for five of 80 samples. Furthermore, the albumin concentrations analyzed by Native PAGE were higher than SDS PAGE in 61 (76.2%) of 80 samples. This study suggests the need for evaluating diabetes not only by HPLC, but also by combining it with another method.

Citing Articles

Usefulness of HPLC assay for early detection of microalbuminuria in chronic kidney disease.

Horikoshi S, Okuda M, Nishimura E, Ohsawa I, Suzuki Y, Shimizu Y J Clin Lab Anal. 2013; 27(4):333-8.

PMID: 23852795 PMC: 6807543. DOI: 10.1002/jcla.21608.

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