High-amylose Wheat Generated by RNA Interference Improves Indices of Large-bowel Health in Rats

Overview

Journal Proc Natl Acad Sci U S A

Specialty Science

Date 2006 Mar 16

PMID 16537443

Citations 121

Authors

Ahmed Regina

Anthony Bird

David Topping

Sarah Bowden

Judy Freeman

Tina Barsby

Behjat Kosar-Hashemi

Zhongyi Li

Sadequr Rahman

Matthew Morell

Affiliations

Soon will be listed here.

Abstract

Foods high in resistant starch have the potential to improve human health and lower the risk of serious noninfectious diseases. RNA interference was used to down-regulate the two different isoforms of starch-branching enzyme (SBE) II (SBEIIa and SBEIIb) in wheat endosperm to raise its amylose content. Suppression of SBEIIb expression alone had no effect on amylose content; however, suppression of both SBEIIa and SBEIIb expression resulted in starch containing >70% amylose. When the >70% amylose wheat grain was fed to rats in a diet as a wholemeal, several indices of large-bowel function, including short-chain fatty acids, were improved relative to standard wholemeal wheat. These results indicate that this high-amylose wheat has a significant potential to improve human health through its resistant starch content.

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