Purification and Characterization of a 1,4-Benzoquinone Reductase from the Basidiomycete Phanerochaete Chrysosporium

Overview

Journal Appl Environ Microbiol

Specialties Environmental Health
Microbiology

Date 1995 Aug 1

PMID 16535104

Citations 23

Authors

B J Brock

S Rieble

M H Gold

Affiliations

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Abstract

An intracellular, soluble 1,4-benzoquinone reductase was purified from agitated cultures of Phanerochaete chrysosporium and characterized. The quinone reductase was expressed in cultures grown under both nitrogen-sufficient and nitrogen-limiting (12 and 1.2 mM ammonium tartrate) conditions. The protein was purified to homogeneity by using ammonium sulfate fractionation, hydrophobic interaction, and ion-exchange and blue-agarose affinity chromatographies. The native flavin mononucleotide-containing protein, pI 4.3, has a molecular mass of 44 kDa as determined by gel filtration. The protein has a subunit molecular mass of ;sim22 kDa as determined by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. The quinone reductase exhibits a broad pH optimum between 5.0 and 6.5 and a temperature optimum of 30(deg)C. The enzyme catalyzes the two-electron reduction of several quinones and other electron acceptors utilizing either NADH or NADPH as an electron donor. The apparent K(infm) for 2-methoxy-1,4-benzoquinone is 2.4 (mu)M, and the apparent k(infcat) is 4.4 x 10(sup5) s(sup-1). Enzyme activity is strongly inhibited by Cibacron blue 3GA and by dicumarol.

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References

Zemper E, BLACK S . Morphology of freeze-etched Treponema refringens (Nichols). Arch Microbiol. 1978; 117(3):227-38. DOI: 10.1007/BF00738540. View

Tien M, Kirk T . Lignin-Degrading Enzyme from the Hymenomycete Phanerochaete chrysosporium Burds. Science. 1983; 221(4611):661-3. DOI: 10.1126/science.221.4611.661. View

Hassan H, Fridovich I . Intracellular production of superoxide radical and of hydrogen peroxide by redox active compounds. Arch Biochem Biophys. 1979; 196(2):385-95. DOI: 10.1016/0003-9861(79)90289-3. View

Buswell J, Hamp S, Eriksson K . Intracellular quinone reduction in Sporotrichum pulverulentum by a NAD(P)H:quinone oxidoreductase: possible role in vanillic acid catabolism. FEBS Lett. 1979; 108(1):229-32. DOI: 10.1016/0014-5793(79)81216-8. View

Entsch B, Ballou D, MASSEY V . Flavin-oxygen derivatives involved in hydroxylation by p-hydroxybenzoate hydroxylase. J Biol Chem. 1976; 251(9):2550-63. View

Valli K, Brock B, Joshi D, Gold M . Degradation of 2,4-dinitrotoluene by the lignin-degrading fungus Phanerochaete chrysosporium. Appl Environ Microbiol. 1992; 58(1):221-8. PMC: 195195. DOI: 10.1128/aem.58.1.221-228.1992. View

Valli K, Wariishi H, Gold M . Degradation of 2,7-dichlorodibenzo-p-dioxin by the lignin-degrading basidiomycete Phanerochaete chrysosporium. J Bacteriol. 1992; 174(7):2131-7. PMC: 205830. DOI: 10.1128/jb.174.7.2131-2137.1992. View

Cadenas E, Hochstein P, Ernster L . Pro- and antioxidant functions of quinones and quinone reductases in mammalian cells. Adv Enzymol Relat Areas Mol Biol. 1992; 65:97-146. DOI: 10.1002/9780470123119.ch3. View

Tuor U, Wariishi H, Schoemaker H, Gold M . Oxidation of phenolic arylglycerol beta-aryl ether lignin model compounds by manganese peroxidase from Phanerochaete chrysosporium: oxidative cleavage of an alpha-carbonyl model compound. Biochemistry. 1992; 31(21):4986-95. DOI: 10.1021/bi00136a011. View

10.

Prestera T, Prochaska H, Talalay P . Inhibition of NAD(P)H:(quinone-acceptor) oxidoreductase by cibacron blue and related anthraquinone dyes: a structure-activity study. Biochemistry. 1992; 31(3):824-33. DOI: 10.1021/bi00118a027. View

11.

Valli K, Gold M . Degradation of 2,4-dichlorophenol by the lignin-degrading fungus Phanerochaete chrysosporium. J Bacteriol. 1991; 173(1):345-52. PMC: 207193. DOI: 10.1128/jb.173.1.345-352.1991. View

12.

Muheim A, Waldner R, Sanglard D, Reiser J, Schoemaker H, Leisola M . Purification and properties of an aryl-alcohol dehydrogenase from the white-rot fungus Phanerochaete chrysosporium. Eur J Biochem. 1991; 195(2):369-75. DOI: 10.1111/j.1432-1033.1991.tb15715.x. View

13.

Wariishi H, Valli K, Gold M . In vitro depolymerization of lignin by manganese peroxidase of Phanerochaete chrysosporium. Biochem Biophys Res Commun. 1991; 176(1):269-75. DOI: 10.1016/0006-291x(91)90919-x. View

14.

Buswell J, Eriksson K . NAD(P)H dehydrogenase (quinone) from Sporotrichum pulverulentum. Methods Enzymol. 1988; 161:271-4. DOI: 10.1016/0076-6879(88)61029-9. View

15.

Kirk T, Farrell R . Enzymatic "combustion": the microbial degradation of lignin. Annu Rev Microbiol. 1987; 41:465-505. DOI: 10.1146/annurev.mi.41.100187.002341. View

16.

Smith P, Krohn R, Hermanson G, Mallia A, Gartner F, Provenzano M . Measurement of protein using bicinchoninic acid. Anal Biochem. 1985; 150(1):76-85. DOI: 10.1016/0003-2697(85)90442-7. View

17.

Faeder E, Siegel L . A rapid micromethod for determination of FMN and FAD in mixtures. Anal Biochem. 1973; 53(1):332-6. DOI: 10.1016/0003-2697(73)90442-9. View

18.

Porter D, Voet J, BRIGHT H . Direct evidence for carbanions and covalent N 5 -flavin-carbanion adducts as catalytic intermediates in the oxidation of nitroethane by D-amino acid oxidase. J Biol Chem. 1973; 248(12):4400-16. View

19.

Iyanagi T, Yamazaki I . One-electron-transfer reactions in biochemical systems. V. Difference in the mechanism of quinone reduction by the NADH dehydrogenase and the NAD(P)H dehydrogenase (DT-diaphorase). Biochim Biophys Acta. 1970; 216(2):282-94. DOI: 10.1016/0005-2728(70)90220-3. View

20.

Laemmli U . Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 1970; 227(5259):680-5. DOI: 10.1038/227680a0. View