Control by Cytochrome C Oxidase of the Cellular Oxidative Phosphorylation System Depends on the Mitochondrial Energy State

Overview

Journal Biochem J

Specialty Biochemistry

Date 2006 Mar 15

PMID 16533168

Citations 36

Authors

Claudia Piccoli

Rosella Scrima

Domenico Boffoli

Nazzareno Capitanio

Affiliations

Soon will be listed here.

Abstract

Recent measurements of the flux control exerted by cytochrome c oxidase on the respiratory activity in intact cells have led to a re-appraisal of its regulatory function. We have further extended this in vivo study in the framework of the Metabolic Control Analysis and evaluated the impact of the mitochondrial transmembrane electrochemical potential (Deltamu(H+)) on the control strength of the oxidase. The results indicate that, under conditions mimicking the mitochondrial State 4 of respiration, both the flux control coefficient and the threshold value of cytochrome oxidase are modified with respect to the uncoupled condition. The results obtained are consistent with a model based on changes in the assembly state of the oxidative phosphorylation enzyme complexes and possible implications in the understanding of exercise-intolerance of human neuromuscular degenerative diseases are discussed.

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