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Computer-controlled Optical Scanning Tile Microscope

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Journal Appl Opt
Date 2006 Mar 10
PMID 16523776
Citations 1
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Abstract

A new type of computer-controlled optical scanning, high-magnification imaging system with a large field of view is described that overcomes the commonly believed incompatibility of achieving both high magnification and a large field of view. The new system incorporates galvanometer scanners, a CCD camera, and a high-brightness LED source for the fast acquisition of a large number of a high-resolution segmented tile images with a magnification of 800x for each tile. The captured segmented tile images are combined to create an effective enlarged view of a target totaling 1.6 mm x 1.2 mm in area. The speed and sensitivity of the system make it suitable for high-resolution imaging and monitoring of a small segmented area of 320 microm x 240 microm with 4 microm resolution. Each tile segment of the target can be zoomed up without loss of the high resolution. This new microscope imaging system gives both high magnification and a large field of view. This microscope can be utilized in medicine, biology, semiconductor inspection, device analysis, and quality control.

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Hayakawa T, Watanabe T, Senoo T, Ishikawa M J Vis Exp. 2017; (122).

PMID: 28448029 PMC: 5564472. DOI: 10.3791/55431.