Mapping Sequences Required for Nuclear Localization and the Transcriptional Activation Function of the Arabidopsis Protein AINTEGUMENTA

Overview

Journal Planta

Specialty Biology

Date 2006 Mar 9

PMID 16523347

Citations 11

Authors

Beth A Krizek

Chidananda Sulli

Affiliations

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Abstract

The Arabidopsis thaliana floral development protein AINTEGUMENTA (ANT) is a member of a large family of DNA binding proteins (AP2/ERF family) that control plant growth and development in response to developmental or environmental signals. Transcriptional activation and/or repression activities have been demonstrated for several members of this protein family. We have used fusions between ANT and the GAL4 DNA binding domain to identify an 80 amino acid sequence important for the transcriptional activation function of ANT. This region shows similarity to transcriptional activation domains in other proteins, as it is rich in Ser/Thr, Gln/Asn, and acidic amino acids. We also demonstrate that ANT can activate gene expression in Arabidopsis plants through binding to a DNA sequence corresponding to an in vitro determined ANT binding site. Finally, we show that ANT is nuclear localized and that the sequence KKKR (amino acids 252-255) is required for nuclear localization of the protein.

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References

Gietz D, St Jean A, Woods R, Schiestl R . Improved method for high efficiency transformation of intact yeast cells. Nucleic Acids Res. 1992; 20(6):1425. PMC: 312198. DOI: 10.1093/nar/20.6.1425. View

Johansen F, Prywes R . Identification of transcriptional activation and inhibitory domains in serum response factor (SRF) by using GAL4-SRF constructs. Mol Cell Biol. 1993; 13(8):4640-7. PMC: 360090. DOI: 10.1128/mcb.13.8.4640-4647.1993. View

Krizek B, Prost V, Macias A . AINTEGUMENTA promotes petal identity and acts as a negative regulator of AGAMOUS. Plant Cell. 2000; 12(8):1357-66. PMC: 149108. DOI: 10.1105/tpc.12.8.1357. View

Nole-Wilson S, Tranby T, Krizek B . AINTEGUMENTA-like (AIL) genes are expressed in young tissues and may specify meristematic or division-competent states. Plant Mol Biol. 2005; 57(5):613-28. DOI: 10.1007/s11103-005-0955-6. View

Vergani P, Morandini P, Soave C . Complementation of a yeast delta pkc1 mutant by the Arabidopsis proteinANT. FEBS Lett. 1997; 400(2):243-6. DOI: 10.1016/s0014-5793(96)01399-3. View

Baker S, Robinson-Beers K, Villanueva J, Gaiser J, Gasser C . Interactions among genes regulating ovule development in Arabidopsis thaliana. Genetics. 1997; 145(4):1109-24. PMC: 1207880. DOI: 10.1093/genetics/145.4.1109. View

Mizukami Y, Fischer R . Plant organ size control: AINTEGUMENTA regulates growth and cell numbers during organogenesis. Proc Natl Acad Sci U S A. 2000; 97(2):942-7. PMC: 15435. DOI: 10.1073/pnas.97.2.942. View

Krizek B, Meyerowitz E . The Arabidopsis homeotic genes APETALA3 and PISTILLATA are sufficient to provide the B class organ identity function. Development. 1996; 122(1):11-22. DOI: 10.1242/dev.122.1.11. View

Triezenberg S . Structure and function of transcriptional activation domains. Curr Opin Genet Dev. 1995; 5(2):190-6. DOI: 10.1016/0959-437x(95)80007-7. View

10.

Liu L, White M, MacRae T . Transcription factors and their genes in higher plants functional domains, evolution and regulation. Eur J Biochem. 1999; 262(2):247-57. DOI: 10.1046/j.1432-1327.1999.00349.x. View

11.

Rieu I, Bots M, Mariani C, Weterings K . Isolation and expression analysis of a tobacco AINTEGUMENTA ortholog (NtANTL). Plant Cell Physiol. 2005; 46(5):803-5. DOI: 10.1093/pcp/pci076. View

12.

Schneitz K, Hulskamp M, Kopczak S, Pruitt R . Dissection of sexual organ ontogenesis: a genetic analysis of ovule development in Arabidopsis thaliana. Development. 1997; 124(7):1367-76. DOI: 10.1242/dev.124.7.1367. View

13.

Liu Z, Franks R, Klink V . Regulation of gynoecium marginal tissue formation by LEUNIG and AINTEGUMENTA. Plant Cell. 2000; 12(10):1879-92. PMC: 149126. DOI: 10.1105/tpc.12.10.1879. View

14.

Klucher K, Chow H, Reiser L, Fischer R . The AINTEGUMENTA gene of Arabidopsis required for ovule and female gametophyte development is related to the floral homeotic gene APETALA2. Plant Cell. 1996; 8(2):137-53. PMC: 161087. DOI: 10.1105/tpc.8.2.137. View

15.

Fujimoto S, Ohta M, Usui A, Shinshi H, Ohme-Takagi M . Arabidopsis ethylene-responsive element binding factors act as transcriptional activators or repressors of GCC box-mediated gene expression. Plant Cell. 2000; 12(3):393-404. PMC: 139839. DOI: 10.1105/tpc.12.3.393. View

16.

Ohta M, Matsui K, Hiratsu K, Shinshi H, Ohme-Takagi M . Repression domains of class II ERF transcriptional repressors share an essential motif for active repression. Plant Cell. 2001; 13(8):1959-68. PMC: 139139. DOI: 10.1105/tpc.010127. View

17.

Elliott R, Betzner A, Huttner E, Oakes M, Tucker W, Gerentes D . AINTEGUMENTA, an APETALA2-like gene of Arabidopsis with pleiotropic roles in ovule development and floral organ growth. Plant Cell. 1996; 8(2):155-68. PMC: 161088. DOI: 10.1105/tpc.8.2.155. View

18.

Krizek B . DNA binding properties of the Arabidopsis floral development protein AINTEGUMENTA. Nucleic Acids Res. 2000; 28(21):4076-82. PMC: 113152. DOI: 10.1093/nar/28.21.4076. View

19.

Krizek B . AINTEGUMENTA utilizes a mode of DNA recognition distinct from that used by proteins containing a single AP2 domain. Nucleic Acids Res. 2003; 31(7):1859-68. PMC: 152808. DOI: 10.1093/nar/gkg292. View

20.

Sridhar V, Surendrarao A, Gonzalez D, Conlan R, Liu Z . Transcriptional repression of target genes by LEUNIG and SEUSS, two interacting regulatory proteins for Arabidopsis flower development. Proc Natl Acad Sci U S A. 2004; 101(31):11494-9. PMC: 509228. DOI: 10.1073/pnas.0403055101. View