Laminin Alpha 1 Gene is Essential for Normal Lens Development in Zebrafish

Overview

Journal BMC Dev Biol

Publisher Biomed Central

Specialties Biology
Reproductive Medicine

Date 2006 Mar 9

PMID 16522196

Citations 26

Authors

Natalya S Zinkevich

Dmitry V Bosenko

Brian A Link

Elena V Semina

Affiliations

Soon will be listed here.

Abstract

Background: Laminins represent major components of basement membranes and play various roles in embryonic and adult tissues. The functional laminin molecule consists of three chains, alpha, beta and gamma, encoded by separate genes. There are twelve different laminin genes identified in mammals to date that are highly homologous in their sequence but different in their tissue distribution. The laminin alpha -1 gene was shown to have the most restricted expression pattern with strong expression in ocular structures, particularly in the developing and mature lens.

Results: We identified the zebrafish lama1 gene encoding a 3075-amino acid protein (lama1) that possesses strong identity with the human LAMA1. Zebrafish lama1 transcripts were detected at all stages of embryo development with the highest levels of expression in the developing lens, somites, nervous and urogenital systems. Translation of the lama1 gene was inhibited using two non-overlapping morpholino oligomers that were complementary to sequences surrounding translation initiation. Morphant embryos exhibited an arrest in lens development and abnormalities in the body axis length and curvature.

Conclusion: These results underline the importance of the laminin alpha 1 for normal ocular development and provide a basis for further analysis of its developmental roles.

Citing Articles

Biologically Relevant Laminin-511 Moderates the Derivation and Proliferation of Human Lens Epithelial Stem/Progenitor-Like Cells.

Li J, Ma J, Chen Y, Chen S, Luo L, Cheng H Invest Ophthalmol Vis Sci. 2024; 65(10):12.

PMID: 39106056 PMC: 11309036. DOI: 10.1167/iovs.65.10.12.

Genome-Wide Identification of Family Related to Follicular Pseudoplacenta Development in Black Rockfish ().

Zhao N, Wang X, Wang T, Xu X, Liu Q, Li J Int J Mol Sci. 2022; 23(18).

PMID: 36142434 PMC: 9504374. DOI: 10.3390/ijms231810523.

High-throughput transcriptome sequencing reveals the key stages of cardiovascular development in zebrafish embryos.

Zhou C, Zhao W, Zhang S, Ma J, Sultan Y, Li X BMC Genomics. 2022; 23(1):587.

PMID: 35964013 PMC: 9375324. DOI: 10.1186/s12864-022-08808-x.

Zebrafish Models of Autosomal Recessive Ataxias.

Quelle-Regaldie A, Sobrido-Camean D, Barreiro-Iglesias A, Sobrido M, Sanchez L Cells. 2021; 10(4).

PMID: 33917666 PMC: 8068028. DOI: 10.3390/cells10040836.

Dynamics of the lens basement membrane capsule and its interaction with connective tissue-like extracapsular matrix proteins.

DeDreu J, Walker J, Menko A Matrix Biol. 2020; 96:18-46.

PMID: 33383103 PMC: 7902460. DOI: 10.1016/j.matbio.2020.12.005.

References

Pollard S, Parsons M, Kamei M, Kettleborough R, Thomas K, Pham V . Essential and overlapping roles for laminin alpha chains in notochord and blood vessel formation. Dev Biol. 2005; 289(1):64-76. DOI: 10.1016/j.ydbio.2005.10.006. View

McLean W, Irvine A, Hamill K, Whittock N, Coleman-Campbell C, Mellerio J . An unusual N-terminal deletion of the laminin alpha3a isoform leads to the chronic granulation tissue disorder laryngo-onycho-cutaneous syndrome. Hum Mol Genet. 2003; 12(18):2395-409. DOI: 10.1093/hmg/ddg234. View

Libby R, Lavallee C, Balkema G, Brunken W, Hunter D . Disruption of laminin beta2 chain production causes alterations in morphology and function in the CNS. J Neurosci. 1999; 19(21):9399-411. PMC: 6782941. View

Falk M, Ferletta M, Forsberg E, Ekblom P . Restricted distribution of laminin alpha1 chain in normal adult mouse tissues. Matrix Biol. 1999; 18(6):557-68. DOI: 10.1016/s0945-053x(99)00047-5. View

Blixt A, Mahlapuu M, Aitola M, Pelto-Huikko M, Enerback S, Carlsson P . A forkhead gene, FoxE3, is essential for lens epithelial proliferation and closure of the lens vesicle. Genes Dev. 2000; 14(2):245-54. PMC: 316354. View

Azuma N, Hirakiyama A, Inoue T, Asaka A, Yamada M . Mutations of a human homologue of the Drosophila eyes absent gene (EYA1) detected in patients with congenital cataracts and ocular anterior segment anomalies. Hum Mol Genet. 2000; 9(3):363-6. DOI: 10.1093/hmg/9.3.363. View

Aso S, Baba R, Noda S, Ikuno S, Fujita M . Hypoplastic basement membrane of the lens anlage in the inheritable lens aplastic mouse (lap mouse). Teratology. 2000; 61(4):262-72. DOI: 10.1002/(SICI)1096-9926(200004)61:4<262::AID-TERA4>3.0.CO;2-9. View

Virtanen I, Gullberg D, Rissanen J, Kivilaakso E, Kiviluoto T, Laitinen L . Laminin alpha1-chain shows a restricted distribution in epithelial basement membranes of fetal and adult human tissues. Exp Cell Res. 2000; 257(2):298-309. DOI: 10.1006/excr.2000.4883. View

Colognato H, Yurchenco P . Form and function: the laminin family of heterotrimers. Dev Dyn. 2000; 218(2):213-34. DOI: 10.1002/(SICI)1097-0177(200006)218:2<213::AID-DVDY1>3.0.CO;2-R. View

10.

Semina E, Murray J, Reiter R, Hrstka R, Graw J . Deletion in the promoter region and altered expression of Pitx3 homeobox gene in aphakia mice. Hum Mol Genet. 2000; 9(11):1575-85. DOI: 10.1093/hmg/9.11.1575. View

11.

Gedde-Dahl Jr T, Dupuy B, Jonassen R, Winberg J, Anton-Lamprecht I, Olaisen B . Junctional epidermolysis bullosa inversa (locus EBR2A) assigned to 1q31 by linkage and association to LAMC1. Hum Mol Genet. 1994; 3(8):1387-91. DOI: 10.1093/hmg/3.8.1387. View

12.

Smith R, Roderick T, Sundberg J . Microphthalmia and associated abnormalities in inbred black mice. Lab Anim Sci. 1994; 44(6):551-60. View

13.

Ljubimov A, Burgeson R, Butkowski R, Michael A, Sun T, Kenney M . Human corneal basement membrane heterogeneity: topographical differences in the expression of type IV collagen and laminin isoforms. Lab Invest. 1995; 72(4):461-73. View

14.

Aso S, Horiwaki S, Noda S . Lens aplasia: a new mutation producing lens abnormality in the mouse. Lab Anim Sci. 1995; 45(1):41-6. View

15.

Zhang X, Topaloglu H, Cruaud C, Tesson F, Weissenbach J, Tome F . Mutations in the laminin alpha 2-chain gene (LAMA2) cause merosin-deficient congenital muscular dystrophy. Nat Genet. 1995; 11(2):216-8. DOI: 10.1038/ng1095-216. View

16.

Stemple D, Zwartkruis F, Neuhauss S, Schier A, Malicki J, Stainier D . Mutations affecting development of the notochord in zebrafish. Development. 1996; 123:117-28. DOI: 10.1242/dev.123.1.117. View

17.

Miner J, Patton B, Lentz S, Gilbert D, Snider W, Jenkins N . The laminin alpha chains: expression, developmental transitions, and chromosomal locations of alpha1-5, identification of heterotrimeric laminins 8-11, and cloning of a novel alpha3 isoform. J Cell Biol. 1997; 137(3):685-701. PMC: 2139892. DOI: 10.1083/jcb.137.3.685. View

18.

Qin P, PIECHOCKI M, Lu S, Kurpakus M . Localization of basement membrane-associated protein isoforms during development of the ocular surface of mouse eye. Dev Dyn. 1997; 209(4):367-76. DOI: 10.1002/(SICI)1097-0177(199708)209:4<367::AID-AJA4>3.0.CO;2-G. View

19.

Sorokin L, Pausch F, Durbeej M, Ekblom P . Differential expression of five laminin alpha (1-5) chains in developing and adult mouse kidney. Dev Dyn. 1998; 210(4):446-62. DOI: 10.1002/(SICI)1097-0177(199712)210:4<446::AID-AJA8>3.0.CO;2-G. View

20.

Brownell I, Dirksen M, Jamrich M . Forkhead Foxe3 maps to the dysgenetic lens locus and is critical in lens development and differentiation. Genesis. 2000; 27(2):81-93. DOI: 10.1002/1526-968x(200006)27:2<81::aid-gene50>3.0.co;2-n. View