Correlation of Activity with Phenotypes of Escherichia Coli Partial Function Mutants of Rnh, the Gene Encoding RNase H

Overview

Journal Mol Gen Genet

Specialties Genetics
Molecular Biology

Date 1991 Jul 1

PMID 1650909

Citations 11

Authors

M Itaya

R J Crouch

Affiliations

Soon will be listed here.

Abstract

The rnh gene of Escherichia coli encodes RNase H. rnh mutants display at least two phenotypes: (1) they require functional RecBCD enzyme for growth; thus rnh-339::cat recB270 (Ts) and rnh-339::cat recC271 (Ts) strains are temperature sensitive for growth; (2) rnh mutants permit replication that is independent of the chromosomal origin, presumably by failing to remove RNA-DNA hybrids from which extra-original replication can be primed. We report here that manifestation of these two phenotypes occurs at different levels of RNase H function; we have examined partially functional rnh mutants for their in vitro RNase H activity, their ability to rescue viability in recB or recC cells and their ability to permit growth of mutants incapable of using oriC [dnaA (Ts)].

Citing Articles

Interaction with single-stranded DNA-binding protein localizes ribonuclease HI to DNA replication forks and facilitates R-loop removal.

Wolak C, Ma H, Soubry N, Sandler S, Reyes-Lamothe R, Keck J Mol Microbiol. 2020; 114(3):495-509.

PMID: 32426857 PMC: 7934204. DOI: 10.1111/mmi.14529.

Integration of stable extracellular DNA released from Escherichia coli into the Bacillus subtilis genome vector by culture mix method.

Itaya M, Kaneko S Nucleic Acids Res. 2010; 38(8):2551-7.

PMID: 20308163 PMC: 2860128. DOI: 10.1093/nar/gkq142.

Depletion of RNase HI activity in Escherichia coli lacking DNA topoisomerase I leads to defects in DNA supercoiling and segregation.

Usongo V, Nolent F, Sanscartier P, Tanguay C, Broccoli S, Baaklini I Mol Microbiol. 2008; 69(4):968-81.

PMID: 18554330 PMC: 2875999. DOI: 10.1111/j.1365-2958.2008.06334.x.

Isolation of RNase H genes that are essential for growth of Bacillus subtilis 168.

Itaya M, Omori A, Kanaya S, Crouch R, Tanaka T, Kondo K J Bacteriol. 1999; 181(7):2118-23.

PMID: 10094689 PMC: 93624. DOI: 10.1128/JB.181.7.2118-2123.1999.

Absence of a direct role for RNase HI in initiation of DNA replication at the oriC site on the Escherichia coli chromosome.

Hong X, Kogoma T J Bacteriol. 1993; 175(20):6731-4.

PMID: 8407851 PMC: 206788. DOI: 10.1128/jb.175.20.6731-6734.1993.

References

Itaya M, McKelvin D, Chatterjie S, Crouch R . Selective cloning of genes encoding RNase H from Salmonella typhimurium, Saccharomyces cerevisiae and Escherichia coli rnh mutant. Mol Gen Genet. 1991; 227(3):438-45. DOI: 10.1007/BF00273935. View

Bockrath R, Wolff L, Farr A, Crouch R . Amplified RNase H activity in Escherichia coli B/r increases sensitivity to ultraviolet radiation. Genetics. 1987; 115(1):33-40. PMC: 1203061. DOI: 10.1093/genetics/115.1.33. View

Lindahl G, Lindahl T . Initiation of DNA replication in Escherichia coli: RNase H-deficient mutants do not require the dnaA function. Mol Gen Genet. 1984; 196(2):283-9. DOI: 10.1007/BF00328061. View

HANSEN E, Atlung T, Hansen F, Skovgaard O, von Meyenburg K . Fine structure genetic map and complementation analysis of mutations in the dnaA gene of Escherichia coli. Mol Gen Genet. 1984; 196(3):387-96. DOI: 10.1007/BF00436184. View

Vanderbilt A, Tessman I . Identification of the altered bases in mutated single-stranded DNA. IV. Nitrous acid induction of the transitions guanine to adenine and thymine to cytosine. Genetics. 1970; 66(1):1-10. PMC: 1212477. DOI: 10.1093/genetics/66.1.1. View

Itaya M, Crouch R . A combination of RNase H (rnh) and recBCD or sbcB mutations in Escherichia coli K12 adversely affects growth. Mol Gen Genet. 1991; 227(3):424-32. DOI: 10.1007/BF00273933. View

Kogoma T . Absence of RNase H allows replication of pBR322 in Escherichia coli mutants lacking DNA polymerase I. Proc Natl Acad Sci U S A. 1984; 81(24):7845-9. PMC: 392249. DOI: 10.1073/pnas.81.24.7845. View

Kogoma T, Subia N, von Meyenburg K . Function of ribonuclease H in initiation of DNA replication in Escherichia coli K-12. Mol Gen Genet. 1985; 200(1):103-9. DOI: 10.1007/BF00383320. View

Torrey T, Atlung T, Kogoma T . dnaA suppressor (dasF) mutants of Escherichia coli are stable DNA replication (sdrA/rnh) mutants. Mol Gen Genet. 1984; 196(2):350-5. DOI: 10.1007/BF00328070. View

10.

Ponticelli A, Schultz D, Taylor A, Smith G . Chi-dependent DNA strand cleavage by RecBC enzyme. Cell. 1985; 41(1):145-51. DOI: 10.1016/0092-8674(85)90069-8. View

11.

Ogawa T, Pickett G, Kogoma T, Kornberg A . RNase H confers specificity in the dnaA-dependent initiation of replication at the unique origin of the Escherichia coli chromosome in vivo and in vitro. Proc Natl Acad Sci U S A. 1984; 81(4):1040-4. PMC: 344759. DOI: 10.1073/pnas.81.4.1040. View

12.

Arendes J, Carl P, Sugino A . A mutation in the rnh-locus of Escherichia coli affects the structural gene for RNase H. Examination of the mutant and wild type protein. J Biol Chem. 1982; 257(9):4719-22. View

13.

Kushner S . In vivo studies of temperature-sensitive recB and recC mutants. J Bacteriol. 1974; 120(3):1213-8. PMC: 245902. DOI: 10.1128/jb.120.3.1213-1218.1974. View

14.

LEVIN J, Crouch R, Post K, Hu S, McKelvin D, ZWEIG M . Functional organization of the murine leukemia virus reverse transcriptase: characterization of a bacterially expressed AKR DNA polymerase deficient in RNase H activity. J Virol. 1988; 62(11):4376-80. PMC: 253878. DOI: 10.1128/JVI.62.11.4376-4380.1988. View

15.

Sasaki M, Fujiyoshi T, Shimada K, Takagi Y . Fine structure of the recB and recC gene region of Escherichia coli. Biochem Biophys Res Commun. 1982; 109(2):414-22. DOI: 10.1016/0006-291x(82)91737-5. View

16.

WITKIN E, Kogoma T . Involvement of the activated form of RecA protein in SOS mutagenesis and stable DNA replication in Escherichia coli. Proc Natl Acad Sci U S A. 1984; 81(23):7539-43. PMC: 392182. DOI: 10.1073/pnas.81.23.7539. View

17.

Tomizawa J, Ogawa H . Structural genes of ATP-dependent deoxyribonuclease of Escherichia coli. Nat New Biol. 1972; 239(88):14-6. DOI: 10.1038/newbio239014a0. View

18.

Martin R, Hearn M, Jenny P, Gallant J . Release factor competition is equivalent at strong and weakly suppressed nonsense codons. Mol Gen Genet. 1988; 213(1):144-9. DOI: 10.1007/BF00333411. View

19.

Ogawa T, Okazaki T . Function of RNase H in DNA replication revealed by RNase H defective mutants of Escherichia coli. Mol Gen Genet. 1984; 193(2):231-7. DOI: 10.1007/BF00330673. View

20.

Lautenberger J, Court D, Papas T . High-level expression in Escherichia coli of the carboxy-terminal sequences of the avian myelocytomatosis virus (MC29) v-myc protein. Gene. 1983; 23(1):75-84. DOI: 10.1016/0378-1119(83)90218-4. View