Crystallization and Preliminary X-ray Analysis of Gene Product 44 from Bacteriophage Mu

Overview

Journal Acta Crystallogr Sect F Struct Biol Cryst Commun

Date 2006 Mar 2

PMID 16508104

Citations 1

Authors

Youhei Kondou

Daisuke Kitazawa

Shigeki Takeda

Eiki Yamashita

Mineyuki Mizuguchi

Keiichi Kawano

Tomitake Tsukihara

Affiliations

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Abstract

Bacteriophage Mu baseplate protein gene product 44 (gp44) is an essential protein required for the assembly of viable phages. To investigate the roles of gp44 in baseplate assembly and infection, gp44 was crystallized at pH 6.0 in the presence of 20% 2-methyl-2,4-pentanediol. The crystals belong to space group R3, with unit-cell parameters a = b = 127.47, c = 63.97 A. The crystals diffract X-rays to at least 2.1 A resolution and are stable in the X-ray beam and are therefore appropriate for structure determination. Native data have been collected to 2.1 A resolution using a DIP6040 image-plate system at beamline BL44XU at the SPring-8 facility in Japan.

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Pukatzki S, Ma A, Revel A, Sturtevant D, Mekalanos J Proc Natl Acad Sci U S A. 2007; 104(39):15508-13.

PMID: 17873062 PMC: 2000545. DOI: 10.1073/pnas.0706532104.

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