Withdrawal from Free-choice Ethanol Consumption Results in Increased Packing Density of Glutamine Synthetase-immunoreactive Astrocytes in the Prelimbic Cortex of Alcohol-preferring Rats

Overview

Journal Alcohol Alcohol

Publisher Oxford University Press

Specialty Psychiatry

Date 2006 Feb 18

PMID 16484281

Citations 28

Authors

Jose Javier Miguel-Hidalgo

Affiliations

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Abstract

Excess activation of glutamatergic neurotransmission in the cerebral cortex following ethanol withdrawal is considered to contribute to significant behavioural disturbances, and to alcohol craving. Astrocytes may play a role in these manifestations because astrocytes are essential in the regulation of released glutamate and its conversion to glutamine through the enzyme glutamine synthetase (GS). However, it is unclear if withdrawal from free-choice ethanol drinking causes changes in the numbers of astrocytes expressing GS or the cytoskeletal protein of astrocytes glial fibrillary acidic protein (GFAP). Alcohol-preferring (P) rats exposed to free-choice ethanol drinking were either maintained without forced interruption of ethanol drinking, subjected to a 3-day withdrawal period at the end of 2 months, or subjected to three 3-day withdrawal periods along 6 months. At 2 months, P rats were also compared with alcohol-naïve alcohol non-preferring rats (NP) rats. Packing density of GS and GFAP-immunoreactive (IR) astrocytes was measured in sections from the prelimbic cortex (PLC) using the optical disector probe. An alcohol deprivation effect was observed in P rats with withdrawals during a 6-month ethanol drinking period. Ethanol withdrawal significantly increased the packing density of GS- and GFAP-IR astrocytes in the PLC of P rats as compared with P rats with continuous access to ethanol. In addition, there was a positive correlation between the pre-withdrawal ethanol consumption and the packing density of GS-IR astrocytes. The present results suggest the involvement of astrocytes in the regulation of the glutamatergic activation associated with withdrawal from free-choice ethanol consumption and point to differential adaptations of GS and GFAP to prolonged alcohol drinking in the PLC of P rats.

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References

De Witte P, Pinto E, Ansseau M, Verbanck P . Alcohol and withdrawal: from animal research to clinical issues. Neurosci Biobehav Rev. 2003; 27(3):189-97. DOI: 10.1016/s0149-7634(03)00030-7. View

Killcross S, Coutureau E . Coordination of actions and habits in the medial prefrontal cortex of rats. Cereb Cortex. 2003; 13(4):400-8. DOI: 10.1093/cercor/13.4.400. View

Uylings H, Groenewegen H, Kolb B . Do rats have a prefrontal cortex?. Behav Brain Res. 2003; 146(1-2):3-17. DOI: 10.1016/j.bbr.2003.09.028. View

Backstrom P, Bachteler D, Koch S, Hyytia P, Spanagel R . mGluR5 antagonist MPEP reduces ethanol-seeking and relapse behavior. Neuropsychopharmacology. 2004; 29(5):921-8. DOI: 10.1038/sj.npp.1300381. View

Rice A, Ross Bullock M, Shelton K . Chronic ethanol consumption transiently reduces adult neural progenitor cell proliferation. Brain Res. 2004; 1011(1):94-8. DOI: 10.1016/j.brainres.2004.01.091. View

Shen F, Chen B, Danias J, Lee K, Lee H, Su Y . Glutamate-induced glutamine synthetase expression in retinal Muller cells after short-term ocular hypertension in the rat. Invest Ophthalmol Vis Sci. 2004; 45(9):3107-12. DOI: 10.1167/iovs.03-0948. View

Nixon K, Crews F . Temporally specific burst in cell proliferation increases hippocampal neurogenesis in protracted abstinence from alcohol. J Neurosci. 2004; 24(43):9714-22. PMC: 6730141. DOI: 10.1523/JNEUROSCI.3063-04.2004. View

Norenberg M, Martinez-Hernandez A . Fine structural localization of glutamine synthetase in astrocytes of rat brain. Brain Res. 1979; 161(2):303-10. DOI: 10.1016/0006-8993(79)90071-4. View

Hallermayer K, Hamprecht B . Cellular heterogeneity in primary cultures of brain cells revealed by immunocytochemical localization of glutamine synthetase. Brain Res. 1984; 295(1):1-11. DOI: 10.1016/0006-8993(84)90810-2. View

10.

Davies D, Vernadakis A . Effects of ethanol on cultured glial cells: proliferation and glutamine synthetase activity. Brain Res. 1984; 318(1):27-35. DOI: 10.1016/0165-3806(84)90059-2. View

11.

Van Eden C, Uylings H . Cytoarchitectonic development of the prefrontal cortex in the rat. J Comp Neurol. 1985; 241(3):253-67. DOI: 10.1002/cne.902410302. View

12.

Van Eden C, Uylings H . Postnatal volumetric development of the prefrontal cortex in the rat. J Comp Neurol. 1985; 241(3):268-74. DOI: 10.1002/cne.902410303. View

13.

Tiffany-Castiglioni E, Roberts J . Reduction of glutamine synthetase specific activity in cultured astroglia by ferrous chloride. J Neurosci Res. 1989; 24(4):508-16. DOI: 10.1002/jnr.490240408. View

14.

Mearow K, Mill J, Freese E . Neuron-glial interactions involved in the regulation of glutamine synthetase. Glia. 1990; 3(5):385-92. DOI: 10.1002/glia.440030510. View

15.

Uylings H, Van Eden C . Qualitative and quantitative comparison of the prefrontal cortex in rat and in primates, including humans. Prog Brain Res. 1990; 85:31-62. DOI: 10.1016/s0079-6123(08)62675-8. View

16.

Hoffman P . Glutamate receptors in alcohol withdrawal-induced neurotoxicity. Metab Brain Dis. 1995; 10(1):73-9. DOI: 10.1007/BF01991784. View

17.

Kane C, Berry A, Boop F, Davies D . Proliferation of astroglia from the adult human cerebrum is inhibited by ethanol in vitro. Brain Res. 1996; 731(1-2):39-44. DOI: 10.1016/0006-8993(96)00456-8. View

18.

Tsai G . Glutamatergic neurotransmission in alcoholism. J Biomed Sci. 1998; 5(5):309-20. DOI: 10.1007/BF02253441. View

19.

McBride W, Li T . Animal models of alcoholism: neurobiology of high alcohol-drinking behavior in rodents. Crit Rev Neurobiol. 1999; 12(4):339-69. DOI: 10.1615/critrevneurobiol.v12.i4.40. View

20.

Rossetti Z, Carboni S, Fadda F . Glutamate-induced increase of extracellular glutamate through N-methyl-D-aspartate receptors in ethanol withdrawal. Neuroscience. 1999; 93(3):1135-40. DOI: 10.1016/s0306-4522(99)00250-x. View