Contrasting Effects of Cd2+ and Co2+ on the Blocking/unblocking of Human Cav3 Channels

Overview

Journal J Membr Biol

Date 2006 Feb 16

PMID 16477530

Citations 8

Authors

D Diaz

R Bartolo

D M Delgadillo

F Higueldo

J C Gomora

Affiliations

Soon will be listed here.

Abstract

Inorganic ions have been used widely to investigate biophysical properties of high voltage-activated calcium channels (HVA: Ca(v)1 and Ca(v)2 families). In contrast, such information regarding low voltage-activated calcium channels (LVA: Ca(v)3 family) is less documented. We have studied the blocking effect of Cd2+, Co2+ and Ni2+ on T-currents expressed by human Ca(v)3 channels: Ca(v)3.1, Ca(v)3.2, and Ca(v)3.3. With the use of the whole-cell configuration of the patch-clamp technique, we have recorded Ca2+ (2 mM: ) currents from HEK-293 cells stably expressing recombinant T-type channels. Cd2+ and Co2+ block was 2- to 3-fold more potent for Ca(v)3.2 channels (EC50 = 65 and 122 microM, respectively) than for the other two LVA channel family members. Current-voltage relationships indicate that Co2+ and Ni2+ shift the voltage dependence of Ca(v)3.1 and Ca(v)3.3 channels activation to more positive potentials. Interestingly, block of those two Ca(v)3 channels by Co2+ and Ni2+ was drastically increased at extreme negative voltages; in contrast, block due to Cd2+ was significantly decreased. This unblocking effect was slightly voltage-dependent. Tail-current analysis reveals a differential effect of Cd2+ on Ca(v)3.3 channels, which can not close while the pore is occupied with this metal cation. The results suggest that metal cations affect differentially T-type channel activity by a mechanism involving the ionic radii of inorganic ions and structural characteristics of the channels pore.

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References

Kim D, Song I, Keum S, Lee T, Jeong M, Kim S . Lack of the burst firing of thalamocortical relay neurons and resistance to absence seizures in mice lacking alpha(1G) T-type Ca(2+) channels. Neuron. 2001; 31(1):35-45. DOI: 10.1016/s0896-6273(01)00343-9. View

Hamill O, Marty A, Neher E, Sakmann B, Sigworth F . Improved patch-clamp techniques for high-resolution current recording from cells and cell-free membrane patches. Pflugers Arch. 1981; 391(2):85-100. DOI: 10.1007/BF00656997. View

Wu J, Lipsius S . Effects of extracellular Mg2+ on T- and L-type Ca2+ currents in single atrial myocytes. Am J Physiol. 1990; 259(6 Pt 2):H1842-50. DOI: 10.1152/ajpheart.1990.259.6.H1842. View

Beedle A, Hamid J, Zamponi G . Inhibition of transiently expressed low- and high-voltage-activated calcium channels by trivalent metal cations. J Membr Biol. 2002; 187(3):225-38. DOI: 10.1007/s00232-001-0166-2. View

Mitterdorfer J, Grabner M, Kraus R, Hering S, Prinz H, Glossmann H . Molecular basis of drug interaction with L-type Ca2+ channels. J Bioenerg Biomembr. 1998; 30(4):319-34. DOI: 10.1023/a:1021933504909. View

Thevenod F, Jones S . Cadmium block of calcium current in frog sympathetic neurons. Biophys J. 1992; 63(1):162-8. PMC: 1262133. DOI: 10.1016/S0006-3495(92)81575-8. View

Chow R . Cadmium block of squid calcium currents. Macroscopic data and a kinetic model. J Gen Physiol. 1991; 98(4):751-70. PMC: 2229079. DOI: 10.1085/jgp.98.4.751. View

Yang J, Ellinor P, Sather W, Zhang J, Tsien R . Molecular determinants of Ca2+ selectivity and ion permeation in L-type Ca2+ channels. Nature. 1993; 366(6451):158-61. DOI: 10.1038/366158a0. View

Takahashi K, Akaike N . Calcium antagonist effects on low-threshold (T-type) calcium current in rat isolated hippocampal CA1 pyramidal neurons. J Pharmacol Exp Ther. 1991; 256(1):169-75. View

10.

Cribbs L, Lee J, Yang J, Satin J, Zhang Y, Daud A . Cloning and characterization of alpha1H from human heart, a member of the T-type Ca2+ channel gene family. Circ Res. 1998; 83(1):103-9. DOI: 10.1161/01.res.83.1.103. View

11.

Wakamori M, Strobeck M, Niidome T, Teramoto T, Imoto K, Mori Y . Functional characterization of ion permeation pathway in the N-type Ca2+ channel. J Neurophysiol. 1998; 79(2):622-34. DOI: 10.1152/jn.1998.79.2.622. View

12.

Cribbs L, Gomora J, Daud A, Lee J, Perez-Reyes E . Molecular cloning and functional expression of Ca(v)3.1c, a T-type calcium channel from human brain. FEBS Lett. 2000; 466(1):54-8. DOI: 10.1016/s0014-5793(99)01756-1. View

13.

Kaneda M, Akaike N . The low-threshold Ca current in isolated amygdaloid neurons in the rat. Brain Res. 1989; 497(1):187-90. DOI: 10.1016/0006-8993(89)90987-6. View

14.

Swandulla D, ARMSTRONG C . Calcium channel block by cadmium in chicken sensory neurons. Proc Natl Acad Sci U S A. 1989; 86(5):1736-40. PMC: 286776. DOI: 10.1073/pnas.86.5.1736. View

15.

Akaike N, Kanaide H, Kuga T, Nakamura M, Sadoshima J, Tomoike H . Low-voltage-activated calcium current in rat aorta smooth muscle cells in primary culture. J Physiol. 1989; 416:141-60. PMC: 1189208. DOI: 10.1113/jphysiol.1989.sp017754. View

16.

Jones S, Marks T . Calcium currents in bullfrog sympathetic neurons. I. Activation kinetics and pharmacology. J Gen Physiol. 1989; 94(1):151-67. PMC: 2228934. DOI: 10.1085/jgp.94.1.151. View

17.

Castelli L, Tanzi F, Taglietti V, Magistretti J . Cu2+, Co2+, and Mn2+ modify the gating kinetics of high-voltage-activated Ca2+ channels in rat palaeocortical neurons. J Membr Biol. 2004; 195(3):121-36. DOI: 10.1007/s00232-003-0614-2. View

18.

Gomora J, Murbartian J, Arias J, Lee J, Perez-Reyes E . Cloning and expression of the human T-type channel Ca(v)3.3: insights into prepulse facilitation. Biophys J. 2002; 83(1):229-41. PMC: 1302142. DOI: 10.1016/s0006-3495(02)75164-3. View

19.

Lee J, Daud A, Cribbs L, Lacerda A, Pereverzev A, Klockner U . Cloning and expression of a novel member of the low voltage-activated T-type calcium channel family. J Neurosci. 1999; 19(6):1912-21. PMC: 6782566. View

20.

Elinder F, Arhem P . Metal ion effects on ion channel gating. Q Rev Biophys. 2004; 36(4):373-427. DOI: 10.1017/s0033583504003932. View