Neutralizing Antibodies to Heterologous Animal Rotavirus Serotypes 5, 6, 7, and 10 in Sera from Ecuadorian Children

Overview

Journal J Clin Microbiol

Specialty Microbiology

Date 1991 May 1

PMID 1647406

Citations 10

Authors

H Brussow

P A Offit

J Sidoti

Affiliations

Soon will be listed here.

Abstract

Serum samples from 870 Ecuadorian children who underwent natural rotavirus exposure were tested for neutralizing serum antibody to heterologous animal rotavirus (RV) serotypes. Six percent of the sera neutralized porcine RV OSU (serotype 5), 10% neutralized bovine RV NCDV (serotype 6), 4% neutralized avian RV Ch-2 (serotype 7), and 8% neutralized bovine RV V1005 (serotype 10). Neutralization was defined as a 90% reduction in infectious virus at a 1:100 serum dilution. The prevalence of antibody to all four heterotypic viruses increased with the age of the children and the number of human RV serotypes neutralized, but prevalences did not differ significantly between children from rural and urban areas of Ecuador. No serum sample that specifically neutralized bovine RV NCDV was identified. We inferred from the seroepidemiological analysis that human RVs contain immunorecessive neutralization epitopes that can stimulate cross-neutralizing antibody to heterotypic animal RVs. This occurs increasingly with age and with the number of human serotypes recognized by a child's neutralizing antibody. Thus, it appears that a broadened immune response to the heterotypic strains occurs with repetitive RV infections.

Citing Articles

Prevalence of neutralizing antibodies against different rotavirus serotypes in children with severe rotavirus-induced diarrhea and their mothers.

Ray P, Kelkar S Clin Diagn Lab Immunol. 2004; 11(1):186-94.

PMID: 14715567 PMC: 321334. DOI: 10.1128/cdli.11.1.186-194.2004.

Glycosphingolipid binding specificities of rotavirus: identification of a sialic acid-binding epitope.

Delorme C, Brussow H, Sidoti J, Roche N, Karlsson K, Neeser J J Virol. 2001; 75(5):2276-87.

PMID: 11160731 PMC: 114811. DOI: 10.1128/JVI.75.5.2276-2287.2001.

Detection of rotavirus types G8 and G10 among Brazilian children with diarrhea.

Santos N, Lima R, Pereira C, Gouvea V J Clin Microbiol. 1998; 36(9):2727-9.

PMID: 9705422 PMC: 105192. DOI: 10.1128/JCM.36.9.2727-2729.1998.

Bovine rotavirus 993/83 shows a third subtype of avian VP7 protein.

Rohwedder A, Hotop H, Minamoto N, Ito H, Nakagomi O, Brussow H Virus Genes. 1997; 14(2):147-51.

PMID: 9237354 DOI: 10.1023/a:1007921418679.

Effect of malnutrition on serum and milk antibodies in Zairian women.

Brussow H, Barclay D, Sidoti J, Rey S, Blondel A, Dirren H Clin Diagn Lab Immunol. 1996; 3(1):37-41.

PMID: 8770501 PMC: 170244. DOI: 10.1128/cdli.3.1.37-41.1996.

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