Complexity in Evolved Regulatory Variation for Alcohol Dehydrogenase Genes in Hawaiian Drosophila

Overview

Journal J Mol Evol

Specialty Biochemistry

Date 1991 Mar 1

PMID 1646337

Citations 7

Authors

X M Fang

C Y Wu

M D Brennan

Affiliations

Soon will be listed here.

Abstract

The alcohol dehydrogenase gene (Adh gene) of Drosophila affinidisjuncta is expressed at a higher level in the larval midgut and Malpighian tubules than the homologous gene from Drosophila hawaiiensis. This study analyzed the cis-acting sequences responsible for these regulatory differences in larval tissues of Drosophila melanogaster transformants. A series of 10 chimeric and deleted Adh genes was introduced into the germ line of D. melanogaster, and tissue-specific expression levels were quantified by gel electrophoresis of tissue extracts. Sequences in the upstream region of the two genes had the strongest influence on enzyme production in the midgut and Malpighian tubules. Other sequence elements also showed effects, some of which were tissue specific. Most gene fragments displayed context-dependent effects, thus supporting the proposed model of polygenic regulation of Adh gene expression.

Citing Articles

Complex organization of promoter and enhancer elements regulate the tissue- and developmental stage-specific expression of the Drosophila melanogaster Gld gene.

Keplinger B, Guo X, Quine J, Feng Y, Cavener D Genetics. 2001; 157(2):699-716.

PMID: 11156990 PMC: 1461511. DOI: 10.1093/genetics/157.2.699.

Polarity of transcriptional enhancement revealed by an insulator element.

Wei W, Brennan M Proc Natl Acad Sci U S A. 2000; 97(26):14518-23.

PMID: 11114171 PMC: 18951. DOI: 10.1073/pnas.011529598.

Redundant cis-acting elements control expression of the Drosophila affinidisjuncta Adh gene in the larval fat body.

McKenzie R, Hu J, Brennan M Nucleic Acids Res. 1994; 22(7):1257-64.

PMID: 8165141 PMC: 523651. DOI: 10.1093/nar/22.7.1257.

Nucleotide sequence of the genomic region encompassing Adh and Adh-dup genes of D. lebanonensis (Scaptodrosophila): gene expression and evolutionary relationships.

Juan E, Papaceit M, Quintana A J Mol Evol. 1994; 38(5):455-67.

PMID: 8028024 DOI: 10.1007/BF00178845.

Wu C, Brennan M Mol Gen Genet. 1993; 240(1):58-64.

PMID: 7688066 DOI: 10.1007/BF00276884.

References

Laurie-Ahlberg C, Stam L . Use of P-element-mediated transformation to identify the molecular basis of naturally occurring variants affecting Adh expression in Drosophila melanogaster. Genetics. 1987; 115(1):129-40. PMC: 1203048. DOI: 10.1093/genetics/115.1.129. View

BENYAJATI C, Place A, Wang N, Pentz E, Sofer W . Deletions at intervening sequence splice sites in the alcohol dehydrogenase gene of Drosophila. Nucleic Acids Res. 1982; 10(22):7261-72. PMC: 327002. DOI: 10.1093/nar/10.22.7261. View

Brennan M, Wu C, Berry A . Tissue-specific regulatory differences for the alcohol dehydrogenase genes of Hawaiian Drosophila are conserved in Drosophila melanogaster transformants. Proc Natl Acad Sci U S A. 1988; 85(18):6866-9. PMC: 282079. DOI: 10.1073/pnas.85.18.6866. View

Rowan R, Dickinson W . Nucleotide sequence of the genomic region encoding alcohol dehydrogenase in Drosophila affinidisjuncta. J Mol Evol. 1988; 28(1-2):43-54. DOI: 10.1007/BF02143496. View

Dickinson W . On the architecture of regulatory systems: evolutionary insights and implications. Bioessays. 1988; 8(6):204-8. DOI: 10.1002/bies.950080608. View

Spradling A, Rubin G . Transposition of cloned P elements into Drosophila germ line chromosomes. Science. 1982; 218(4570):341-7. DOI: 10.1126/science.6289435. View

Steller H, Pirrotta V . P transposons controlled by the heat shock promoter. Mol Cell Biol. 1986; 6(5):1640-9. PMC: 367691. DOI: 10.1128/mcb.6.5.1640-1649.1986. View

Mills L, Batterham P, Alegre J, Starmer W, Sullivan D . Molecular genetic characterization of a locus that contains duplicate Adh genes in Drosophila mojavensis and related species. Genetics. 1986; 112(2):295-310. PMC: 1202702. DOI: 10.1093/genetics/112.2.295. View

Batterham P, Chambers G, Starmer W, Sullivan D . ORIGIN AND EXPRESSION OF AN ALCOHOL DEHYDROGENASE GENE DUPLICATION IN THE GENUS DROSOPHILA. Evolution. 2017; 38(3):644-657. DOI: 10.1111/j.1558-5646.1984.tb00331.x. View

10.

Sofer W, Ursprung H . Drosophila alcohol dehydrogenase. Purification and partial characterization. J Biol Chem. 1968; 243(11):3110-5. View

11.

Rubin G, Spradling A . Vectors for P element-mediated gene transfer in Drosophila. Nucleic Acids Res. 1983; 11(18):6341-51. PMC: 326377. DOI: 10.1093/nar/11.18.6341. View

12.

Brennan M, Rowan R, Rabinow L, Dickinson W . Isolation and initial characterization of the alcohol dehydrogenase gene from Drosophila affinidisjuncta. J Mol Appl Genet. 1984; 2(5):436-46. View

13.

Rabinow L, Dickinson W . Complex cis-acting regulators and locus structure of Drosophila tissue-specific ADH variants. Genetics. 1986; 112(3):523-37. PMC: 1202762. DOI: 10.1093/genetics/112.3.523. View

14.

Fischer J, Maniatis T . Drosophila Adh: a promoter element expands the tissue specificity of an enhancer. Cell. 1988; 53(3):451-61. DOI: 10.1016/0092-8674(88)90165-1. View

15.

Rowan R, Brennan M, Dickinson W . Developmentally regulated RNA transcripts coding for alcohol dehydrogenase in Drosophila affinidisjuncta. Genetics. 1986; 114(2):405-33. PMC: 1202948. DOI: 10.1093/genetics/114.2.405. View

16.

Rubin G, Spradling A . Genetic transformation of Drosophila with transposable element vectors. Science. 1982; 218(4570):348-53. DOI: 10.1126/science.6289436. View

17.

Wu C, Mote Jr J, Brennan M . Tissue-specific expression phenotypes of Hawaiian Drosophila Adh genes in Drosophila melanogaster transformants. Genetics. 1990; 125(3):599-610. PMC: 1204086. DOI: 10.1093/genetics/125.3.599. View

18.

Rowan R, Dickinson W . Two alternative transcripts coding for alcohol dehydrogenase accumulate with different developmental specificities in different species of picture-winged Drosophila. Genetics. 1986; 114(2):435-52. PMC: 1202949. DOI: 10.1093/genetics/114.2.435. View

19.

Corbin V, Maniatis T . Identification of cis-regulatory elements required for larval expression of the Drosophila melanogaster alcohol dehydrogenase gene. Genetics. 1990; 124(3):637-46. PMC: 1203957. DOI: 10.1093/genetics/124.3.637. View

20.

Southern E . Detection of specific sequences among DNA fragments separated by gel electrophoresis. J Mol Biol. 1975; 98(3):503-17. DOI: 10.1016/s0022-2836(75)80083-0. View