Uteroglobin-related Protein 1 Expression Suppresses Allergic Airway Inflammation in Mice

Overview

Journal Am J Respir Crit Care Med

Specialty Critical Care

Date 2006 Feb 4

PMID 16456148

Citations 49

Authors

Yoshihiko Chiba

Reiko Kurotani

Takashi Kusakabe

Tomiko Miura

Benjamin W Link

Miwa Misawa

Shioko Kimura

Affiliations

Soon will be listed here.

Abstract

Rationale: Uteroglobin-related protein (UGRP) 1, which is highly expressed in the epithelial cells of the airways, has been suggested to play a role in lung inflammation.

Objectives: The aim of study was to understand the effect of overexpressed UGRP1 on lung inflammation in a mouse model of allergic airway inflammation.

Methods: Ovalbumin-sensitized and -challenged mice, a model for allergic airway inflammation, were used in conjunction with recombinant adenovirus expressing UGRP1.

Measurements And Main Results: We demonstrated that intranasal administration of adeno-UGRP1 successfully delivered UGRP1 to the epithelial cells of airways and markedly reduced the number of infiltrating inflammatory cells, particularly eosinophils, in lung tissue as well as the level of proinflammatory cytokines such as interleukin (IL)-4, IL-5, and IL-13 in bronchoalveolar lavage fluids. The healed phase of inflammation was clearly seen in the peripheral areas of adeno-UGRP1-treated mouse lungs.

Conclusion: These results demonstrate that UGRP1 can suppress inflammation in the mouse model of allergic airway inflammation. Based on this result, we propose UGRP1 as a novel therapeutic candidate for treating lung inflammation such as is found in asthma.

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References

Trifilieff A, Bertrand C . Time course of inflammatory and remodeling events in a murine model of asthma: effect of steroid treatment. Am J Physiol Lung Cell Mol Physiol. 2000; 279(6):L1120-8. DOI: 10.1152/ajplung.2000.279.6.L1120. View

Muruve D . The innate immune response to adenovirus vectors. Hum Gene Ther. 2005; 15(12):1157-66. DOI: 10.1089/hum.2004.15.1157. View

Klug J, Beier H, Bernard A, Chilton B, Fleming T, Lehrer R . Uteroglobin/Clara cell 10-kDa family of proteins: nomenclature committee report. Ann N Y Acad Sci. 2001; 923:348-54. DOI: 10.1111/j.1749-6632.2000.tb05549.x. View

Niimi T, Popescu N, Zhou Y, Levitt R, Kimura S . UGRP1, a uteroglobin/Clara cell secretory protein-related protein, is a novel lung-enriched downstream target gene for the T/EBP/NKX2.1 homeodomain transcription factor. Mol Endocrinol. 2001; 15(11):2021-36. DOI: 10.1210/mend.15.11.0728. View

Niimi T, Munakata M, Keck-Waggoner C, Popescu N, Levitt R, Hisada M . A polymorphism in the human UGRP1 gene promoter that regulates transcription is associated with an increased risk of asthma. Am J Hum Genet. 2002; 70(3):718-25. PMC: 384948. DOI: 10.1086/339272. View

Cheng G, Arima M, Honda K, Hirata H, Eda F, Yoshida N . Anti-interleukin-9 antibody treatment inhibits airway inflammation and hyperreactivity in mouse asthma model. Am J Respir Crit Care Med. 2002; 166(3):409-16. DOI: 10.1164/rccm.2105079. View

Niimi T, Copeland N, Gilbert D, Jenkins N, Srisodsai A, Zimonjic D . Cloning, expression, and chromosomal localization of the mouse gene (Scgb3a1, alias Ugrp2) that encodes a member of the novel uteroglobin-related protein gene family. Cytogenet Genome Res. 2002; 97(1-2):120-7. DOI: 10.1159/000064067. View

Liu Q, Muruve D . Molecular basis of the inflammatory response to adenovirus vectors. Gene Ther. 2003; 10(11):935-40. DOI: 10.1038/sj.gt.3302036. View

Bin L, Nielson L, Liu X, Mason R, Shu H . Identification of uteroglobin-related protein 1 and macrophage scavenger receptor with collagenous structure as a lung-specific ligand-receptor pair. J Immunol. 2003; 171(2):924-30. DOI: 10.4049/jimmunol.171.2.924. View

10.

Jian Z, Nakayama J, Noguchi E, Shibasaki M, Arinami T . No evidence for association between the -112G/A polymorphism of UGRP1 and childhood atopic asthma. Clin Exp Allergy. 2003; 33(7):902-4. DOI: 10.1046/j.1365-2222.2003.01703.x. View

11.

Heinzmann A, Dietrich H, Deichmann K . Association of uteroglobulin-related protein 1 with bronchial asthma. Int Arch Allergy Immunol. 2003; 131(4):291-5. DOI: 10.1159/000072141. View

12.

Pouladi M, Robbins C, Swirski F, Cundall M, McKenzie A, Jordana M . Interleukin-13-dependent expression of matrix metalloproteinase-12 is required for the development of airway eosinophilia in mice. Am J Respir Cell Mol Biol. 2003; 30(1):84-90. DOI: 10.1165/rcmb.2003-0051OC. View

13.

Cho J, Miller M, Baek K, Han J, Nayar J, Lee S . Inhibition of airway remodeling in IL-5-deficient mice. J Clin Invest. 2004; 113(4):551-60. PMC: 338264. DOI: 10.1172/JCI19133. View

14.

Mandal A, Zhang Z, Ray R, Choi M, Chowdhury B, Pattabiraman N . Uteroglobin represses allergen-induced inflammatory response by blocking PGD2 receptor-mediated functions. J Exp Med. 2004; 199(10):1317-30. PMC: 2211805. DOI: 10.1084/jem.20031666. View

15.

Postma D, Bleecker E, Amelung P, Holroyd K, Xu J, Panhuysen C . Genetic susceptibility to asthma--bronchial hyperresponsiveness coinherited with a major gene for atopy. N Engl J Med. 1995; 333(14):894-900. DOI: 10.1056/NEJM199510053331402. View

16.

Gavett S, OHearn D, Karp C, Patel E, Schofield B, Finkelman F . Interleukin-4 receptor blockade prevents airway responses induced by antigen challenge in mice. Am J Physiol. 1997; 272(2 Pt 1):L253-61. DOI: 10.1152/ajplung.1997.272.2.L253. View

17.

Ohkawara Y, Lei X, Stampfli M, Marshall J, Xing Z, Jordana M . Cytokine and eosinophil responses in the lung, peripheral blood, and bone marrow compartments in a murine model of allergen-induced airways inflammation. Am J Respir Cell Mol Biol. 1997; 16(5):510-20. DOI: 10.1165/ajrcmb.16.5.9160833. View

18.

Singh G, Katyal S . Clara cells and Clara cell 10 kD protein (CC10). Am J Respir Cell Mol Biol. 1997; 17(2):141-3. DOI: 10.1165/ajrcmb.17.2.f138. View

19.

Teramoto S, Matsuse T, Oka T, Ito H, Fukuchi Y, Ouchi Y . Investigation of effects of anesthesia and age on aspiration in mice through LacZ gene transfer by recombinant E1-deleted adenovirus vectors. Am J Respir Crit Care Med. 1998; 158(6):1914-9. DOI: 10.1164/ajrccm.158.6.9801127. View

20.

Chiba Y, Kusakabe T, Kimura S . Decreased expression of uteroglobin-related protein 1 in inflamed mouse airways is mediated by IL-9. Am J Physiol Lung Cell Mol Physiol. 2004; 287(6):L1193-8. DOI: 10.1152/ajplung.00263.2004. View