Molecular Organization of the Maternal Effect Region of the Shaker Complex of Drosophila: Characterization of an I(A) Channel Transcript with Homology to Vertebrate Na Channel

Overview

Journal EMBO J

Specialties Biotechnology
Molecular Biology

Date 1987 Nov 1

PMID 16453805

Citations 42

Authors

A Baumann

I Krah-Jentgens

R Muller

F Muller-Holtkamp

R Seidel

N Kecskemethy

J Casal

A Ferrus

O Pongs

Affiliations

Soon will be listed here.

Abstract

We have cloned 215-kb DNA containing the maternal effect region (ME) of the Shaker gene complex (shC) at 16F of the Drosophila X chromosome. Five translocation and deletion breakpoints have been mapped on the cloned DNA allowing a correlation of the genetic map to transcription units. The ME region spans 100 kb. The genetic behavior of this region correlates with the occurrence of maternal RNAs in this part of the ShC. Two transcripts have been identified in the vicinity of chromosomal rearrangements which cause a Sh phenotype. These are a 4.5-kb transcript interrupted by T(x;2)B27 and a 2-kb transcript interrupted by T(X;3)Sh and T(X;Y)W32. The latter transcript is derived from a primary transcript which spans >65 kb genomic DNA. The cDNA-sequencing data show that this Shaker (I(A)channel) gene can encode a protein of 35 kd with three alpha-helical membrane-spanning sequences near its carboxyl terminus. These have a striking homology with membrane-spanning sequences of the vertabrate Na channel.

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References

Tanouye M, Ferrus A, Fujita S . Abnormal action potentials associated with the Shaker complex locus of Drosophila. Proc Natl Acad Sci U S A. 1981; 78(10):6548-52. PMC: 349078. DOI: 10.1073/pnas.78.10.6548. View

SANGER F, Nicklen S, Coulson A . DNA sequencing with chain-terminating inhibitors. Proc Natl Acad Sci U S A. 1977; 74(12):5463-7. PMC: 431765. DOI: 10.1073/pnas.74.12.5463. View

Maniatis T, Hardison R, Lacy E, Lauer J, OConnell C, Quon D . The isolation of structural genes from libraries of eucaryotic DNA. Cell. 1978; 15(2):687-701. DOI: 10.1016/0092-8674(78)90036-3. View

Grunstein M, Hogness D . Colony hybridization: a method for the isolation of cloned DNAs that contain a specific gene. Proc Natl Acad Sci U S A. 1975; 72(10):3961-5. PMC: 433117. DOI: 10.1073/pnas.72.10.3961. View

Wu C, Haugland F . Voltage clamp analysis of membrane currents in larval muscle fibers of Drosophila: alteration of potassium currents in Shaker mutants. J Neurosci. 1985; 5(10):2626-40. PMC: 6565151. View

Tanouye M, Kamb C, Iverson L, Salkoff L . Genetics and molecular biology of ionic channels in Drosophila. Annu Rev Neurosci. 1986; 9:255-76. DOI: 10.1146/annurev.ne.09.030186.001351. View

Brandl C, Deber C . Hypothesis about the function of membrane-buried proline residues in transport proteins. Proc Natl Acad Sci U S A. 1986; 83(4):917-21. PMC: 322981. DOI: 10.1073/pnas.83.4.917. View

Jan Y, Jan L, Dennis M . Two mutations of synaptic transmission in Drosophila. Proc R Soc Lond B Biol Sci. 1977; 198(1130):87-108. DOI: 10.1098/rspb.1977.0087. View

Potter S, Brorein Jr W, Dunsmuir P, Rubin G . Transposition of elements of the 412, copia and 297 dispersed repeated gene families in Drosophila. Cell. 1979; 17(2):415-27. DOI: 10.1016/0092-8674(79)90168-5. View

10.

Noda M, Ikeda T, Kayano T, Suzuki H, Takeshima H, Kurasaki M . Existence of distinct sodium channel messenger RNAs in rat brain. Nature. 1986; 320(6058):188-92. DOI: 10.1038/320188a0. View

11.

Tanouye M, Ferrus A . Action potentials in normal and Shaker mutant Drosophila. J Neurogenet. 1985; 2(4):253-71. DOI: 10.3109/01677068509102322. View

12.

Hogness D, Lipshitz H, Beachy P, Peattie D, Saint R, Goldschmidt-Clermont M . Regulation and products of the Ubx domain of the bithorax complex. Cold Spring Harb Symp Quant Biol. 1985; 50:181-94. DOI: 10.1101/sqb.1985.050.01.024. View

13.

Brennan M, Rowan R, Dickinson W . Introduction of a functional P element into the germ-line of Drosophila hawaiiensis. Cell. 1984; 38(1):147-51. DOI: 10.1016/0092-8674(84)90535-x. View

14.

Thomas P . Hybridization of denatured RNA and small DNA fragments transferred to nitrocellulose. Proc Natl Acad Sci U S A. 1980; 77(9):5201-5. PMC: 350025. DOI: 10.1073/pnas.77.9.5201. View

15.

Popot J, Changeux J . Nicotinic receptor of acetylcholine: structure of an oligomeric integral membrane protein. Physiol Rev. 1984; 64(4):1162-239. DOI: 10.1152/physrev.1984.64.4.1162. View

16.

Noda M, Shimizu S, Tanabe T, Takai T, Kayano T, Ikeda T . Primary structure of Electrophorus electricus sodium channel deduced from cDNA sequence. Nature. 1984; 312(5990):121-7. DOI: 10.1038/312121a0. View

17.

Adams D, Smith S, Thompson S . Ionic currents in molluscan soma. Annu Rev Neurosci. 1980; 3:141-67. DOI: 10.1146/annurev.ne.03.030180.001041. View

18.

Salkoff L, WYMAN R . Genetic modification of potassium channels in Drosophila Shaker mutants. Nature. 1981; 293(5829):228-30. DOI: 10.1038/293228a0. View

19.

Wu C, Ganetzky B, Haugland F, Liu A . Potassium currents in Drosophila: different components affected by mutations of two genes. Science. 1983; 220(4601):1076-8. DOI: 10.1126/science.6302847. View

20.

Koto M, Tanouye M, Ferrus A, Thomas J, WYMAN R . The morphology of the cervical giant fiber neuron of Drosophila. Brain Res. 1981; 221(2):213-7. DOI: 10.1016/0006-8993(81)90772-1. View