Variable DNA Splicing Sites of a Mitochondrial Intron: Relationship to the Senescence Process in Podospora

Overview

Journal EMBO J

Specialties Biotechnology
Molecular Biology

Date 1986 Mar 1

PMID 16453676

Citations 13

Authors

L Belcour

C Vierny

Affiliations

Soon will be listed here.

Abstract

The unavoidable phenomenon of senescence in Podospora was previously shown to be correlated with the presence of a senescence-specific DNA originating from amplification of some regions of the mitochondrial chromosome. The most frequently amplified region (alpha) corresponds to the first intron of the gene coding for subunit one of cytochrome oxidase. Eleven long-lived mitochondrial mutants were isolated. Here we report sequencing experiments that show that three of them are deleted for most of intron alpha and for a few base pairs belonging to the upstream adjacent exon. We also report an analysis of the residual mitochondrial DNA associated with amplification of senescence-specific DNA alpha which allows us to identify, in senescent cultures, mitochondrial chromosomes lacking sequence alpha. These results taken together suggest that excision of intron alpha from the mitochondrial DNA occurs systematically during the aging process in Podospora. They furthermore provide the first example of inaccurate intron excision at the DNA level.

Citing Articles

Podospora anserina: a model organism to study mechanisms of healthy ageing.

Scheckhuber C, Osiewacz H Mol Genet Genomics. 2008; 280(5):365-74.

PMID: 18797929 DOI: 10.1007/s00438-008-0378-6.

Insertion of short poly d(A) d(T) sequences at recombination junctions in mitochondrial DNA of Podospora.

Koll F, Begel O, Belcour L Mol Gen Genet. 1987; 209(3):630-2.

PMID: 17193717 DOI: 10.1007/BF00331176.

A causal link between respiration and senescence in Podospora anserina.

Dufour E, Boulay J, Rincheval V, Sainsard-Chanet A Proc Natl Acad Sci U S A. 2000; 97(8):4138-43.

PMID: 10759557 PMC: 18174. DOI: 10.1073/pnas.070501997.

Mitochondrial group II introns, cytochrome c oxidase, and senescence in Podospora anserina.

Begel O, Boulay J, Albert B, Dufour E, Sainsard-Chanet A Mol Cell Biol. 1999; 19(6):4093-100.

PMID: 10330149 PMC: 104368. DOI: 10.1128/MCB.19.6.4093.

Three mitochondrial unassigned open reading frames of Podospora anserina represent remnants of a viral-type RNA polymerase gene.

Hermanns J, Osiewacz H Curr Genet. 1994; 25(2):150-7.

PMID: 8087884 DOI: 10.1007/BF00309541.

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