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An Agrobacterium-transformed Cell Culture from the Monocot Asparagus Officinalis

Overview
Journal EMBO J
Specialties Biotechnology
Molecular Biology
Date 1984 Dec 20
PMID 16453585
Citations 43
Authors
J P Hernalsteens
L Thia-Toong
J Schell
M Van Montagu
Affiliations
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Abstract

Cultured stem fragments from the monocotyledonous plant Asparagus officinalis infected by the oncogenic bacterium Agrobacterium tumefaciens developed tumorous proliferations. This tissue was propagated in vitro on hormone-free culture medium. The T-DNA-encoded markers nopaline and agrocinopine were unambiguously detected in these tissues. The data demonstrate that stable T-DNA transfer as well as expression of T-DNA genes is possible in at least some monocotyledonous plants. This opens new possibilities for plant genetic engineering using the Ti plasmid as a gene vector.

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