The DNA Sequence Analysis of Soybean Heat-shock Genes and Identification of Possible Regulatory Promoter Elements

Overview

Journal EMBO J

Specialties Biotechnology
Molecular Biology

Date 1984 Nov 1

PMID 16453563

Citations 50

Authors

F Schoffl

E Raschke

R T Nagao

Affiliations

Soon will be listed here.

Abstract

The soybean possesses a gene family encoding the major low mol. wt. heat-shock proteins of 15-18 kd. We have determined the primary DNA sequences of two of the genes, both located on the same subgenomic DNA fragment. The protein coding regions are characterized by long uninterrupted open reading frames and by sequence homology of 92% and 100% with a heat-shock specific cDNA. One protein sequence deduced from the completely cloned gene hs6871 is composed of 153 amino acids with a total mol. wt. of 17.3 kd; the other protein is a truncated polypeptide containing 73 amino acids at the carboxy-terminal end of an incompletely cloned heat-shock gene designated hs6834. Investigations of the hydrophilic/hydrophobic characteristics of the polypeptides revealed a conservation of structural features between heat-shock proteins from soybean, Caenorhabditis and Drosophila and mammalian lens alpha-crystallin. The 5' end of the soybean heat-shock gene hs6871 was mapped by S1 nuclease at a position which is 100 nucleotides upstream from the translation start codon and 25 nucleotides downstream from a TATA-box sequence. Six other potential promoter elements which are homologous to the Drosophila heat-shock consensus sequence CT-GAA-TTC-AG-, are present within 150 nucleotides upstream from the TATA-box. The possible functions of these promoter elements in transcriptional regulation of expression of soybean heat-shock gene are discussed.

Citing Articles

An Engineered Heat-Inducible Expression System for the Production of Casbene in .

Forestier E, Cording A, Loake G, Graham I Int J Mol Sci. 2023; 24(14).

PMID: 37511181 PMC: 10379985. DOI: 10.3390/ijms241411425.

Development of microfluidic chip for entrapping tobacco BY-2 cells.

Shimizu K, Kawakatsu Y, Kurotani K, Kikkawa M, Tabata R, Kurihara D PLoS One. 2022; 17(4):e0266982.

PMID: 35421187 PMC: 9009702. DOI: 10.1371/journal.pone.0266982.

ARR17 controls dioecy in by repressing B-class MADS-box gene expression.

Leite Montalvao A, Kersten B, Kim G, Fladung M, Muller N Philos Trans R Soc Lond B Biol Sci. 2022; 377(1850):20210217.

PMID: 35306887 PMC: 8935312. DOI: 10.1098/rstb.2021.0217.

Level of tissue differentiation influences the activation of a heat-inducible flower-specific system for genetic containment in poplar (Populus tremula L.).

Hoenicka H, Lehnhardt D, Nunna S, Reinhardt R, Jeltsch A, Briones V Plant Cell Rep. 2015; 35(2):369-84.

PMID: 26521210 DOI: 10.1007/s00299-015-1890-x.

Generation of a homozygous fertilization-defective gcs1 mutant by heat-inducible removal of a rescue gene.

Nagahara S, Takeuchi H, Higashiyama T Plant Reprod. 2015; 28(1):33-46.

PMID: 25673573 PMC: 4333230. DOI: 10.1007/s00497-015-0256-4.

References

Vieira J, Messing J . The pUC plasmids, an M13mp7-derived system for insertion mutagenesis and sequencing with synthetic universal primers. Gene. 1982; 19(3):259-68. DOI: 10.1016/0378-1119(82)90015-4. View

Brisson N, Verma D . Soybean leghemoglobin gene family: normal, pseudo, and truncated genes. Proc Natl Acad Sci U S A. 1982; 79(13):4055-9. PMC: 346575. DOI: 10.1073/pnas.79.13.4055. View

Wu C . Two protein-binding sites in chromatin implicated in the activation of heat-shock genes. Nature. 1984; 309(5965):229-34. DOI: 10.1038/309229a0. View

Pelham H, Bienz M . A synthetic heat-shock promoter element confers heat-inducibility on the herpes simplex virus thymidine kinase gene. EMBO J. 1982; 1(11):1473-7. PMC: 553234. DOI: 10.1002/j.1460-2075.1982.tb01340.x. View

Dennis E, Gerlach W, Pryor A, Bennetzen J, Inglis A, Llewellyn D . Molecular analysis of the alcohol dehydrogenase (Adh1) gene of maize. Nucleic Acids Res. 1984; 12(9):3983-4000. PMC: 318805. DOI: 10.1093/nar/12.9.3983. View

Nagao R, Shah D, Eckenrode V, Meagher R . Multigene family of actin-related sequences isolated from a soybean genomic library. DNA. 1981; 1(1):1-9. DOI: 10.1089/dna.1.1981.1.1. View

Ingolia T, Craig E, McCarthy B . Sequence of three copies of the gene for the major Drosophila heat shock induced protein and their flanking regions. Cell. 1980; 21(3):669-79. DOI: 10.1016/0092-8674(80)90430-4. View

Karch F, Torok I, TISSIERES A . Extensive regions of homology in front of the two hsp70 heat shock variant genes in Drosophila melanogaster. J Mol Biol. 1981; 148(3):219-30. DOI: 10.1016/0022-2836(81)90536-2. View

Pelham H . A regulatory upstream promoter element in the Drosophila hsp 70 heat-shock gene. Cell. 1982; 30(2):517-28. DOI: 10.1016/0092-8674(82)90249-5. View

10.

Schoffl F, Key J . An analysis of mRNAs for a group of heat shock proteins of soybean using cloned cDNAs. J Mol Appl Genet. 1982; 1(4):301-14. View

11.

Proudfoot N . The end of the message. Nature. 1982; 298(5874):516-7. DOI: 10.1038/298516a0. View

12.

Pedersen K, Devereux J, Wilson D, Sheldon E, Larkins B . Cloning and sequence analysis reveal structural variation among related zein genes in maize. Cell. 1982; 29(3):1015-26. DOI: 10.1016/0092-8674(82)90465-2. View

13.

Key J, Lin C, Chen Y . Heat shock proteins of higher plants. Proc Natl Acad Sci U S A. 1981; 78(6):3526-30. PMC: 319602. DOI: 10.1073/pnas.78.6.3526. View

14.

Lin C, Roberts J, Key J . Acquisition of Thermotolerance in Soybean Seedlings : Synthesis and Accumulation of Heat Shock Proteins and their Cellular Localization. Plant Physiol. 1984; 74(1):152-60. PMC: 1066642. DOI: 10.1104/pp.74.1.152. View

15.

Garnier J, Osguthorpe D, Robson B . Analysis of the accuracy and implications of simple methods for predicting the secondary structure of globular proteins. J Mol Biol. 1978; 120(1):97-120. DOI: 10.1016/0022-2836(78)90297-8. View

16.

van der Ouderaa F, de Jong W, Hilderink A, Bloemendal H . The amino-acids sequence of the alphaB2 chain of bovine alpha-crystallin. Eur J Biochem. 1974; 49(1):157-68. DOI: 10.1111/j.1432-1033.1974.tb03821.x. View

17.

Darnell Jr J . Variety in the level of gene control in eukaryotic cells. Nature. 1982; 297(5865):365-71. DOI: 10.1038/297365a0. View

18.

Benoist C, OHare K, Breathnach R, Chambon P . The ovalbumin gene-sequence of putative control regions. Nucleic Acids Res. 1980; 8(1):127-42. PMC: 327247. DOI: 10.1093/nar/8.1.127. View

19.

Voellmy R, Goldschmidt-Clermont M, Southgate R, TISSIERES A, Levis R, Gehring W . A DNA segment isolated from chromosomal site 67B in D. melanogaster contains four closely linked heat-shock genes. Cell. 1981; 23(1):261-70. DOI: 10.1016/0092-8674(81)90290-7. View

20.

Pustell J, Kafatos F . A convenient and adaptable package of DNA sequence analysis programs for microcomputers. Nucleic Acids Res. 1982; 10(1):51-9. PMC: 326113. DOI: 10.1093/nar/10.1.51. View