A Transformation System for the Hypotrichous Ciliate Stylonychia Mytilus

Overview

Journal EMBO J

Specialties Biotechnology
Molecular Biology

Date 1983 Jan 1

PMID 16453475

Citations 10

Authors

I U Wunning

H J Lipps

Affiliations

Soon will be listed here.

Abstract

We describe a transformation system for the ciliate Stylonychia mytilus. The neomycin resistance gene from Escherichia coli transposon Tn5, which codes for the enzyme phosphotransferase and confers resistance to the antibiotic G 418, was ligated into macronuclear ;gene-size' DNA molecules. Using this recombinant DNA for transformation experiments we show that the gene is replicated and expressed in transformed cells.

Citing Articles

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The organization of internal telomeric repeats in the polytene chromosomes of the hypotrichous ciliate Stylonychia lemnae.

Stoll S, Zirlik T, Maercker C, Lipps H Nucleic Acids Res. 1993; 21(8):1783-8.

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Transformation of Tetrahymena thermophila by microinjection of ribosomal RNA genes.

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