Molecular Analysis of Large Transposable Elements Carrying the White Locus of Drosophila Melanogaster

Overview

Journal EMBO J

Specialties Biotechnology
Molecular Biology

Date 1983 Jan 1

PMID 16453456

Citations 21

Authors

R Paro

M L Goldberg

W J Gehring

Affiliations

Soon will be listed here.

Abstract

A large transposable element (TE) comprising the white-apricot and roughest genes has been found to transpose to well over a hundred sites scattered over the Drosophila genome. We report the cloning of the essential parts of several TEs. TE98 and TE28 sequences were cloned by ;walking' along the chromosome from the previously cloned heatshock genes. The ends of the TEs are characterized by dispersed repetitive elements belonging to the foldback (FB) family. FB elements are also associated with two independently isolated transposable elements originating from the white locus, Tp w-1 and Tp w. The strong correlation between FB elements and large composite transposons suggests that a pair of these elements can mobilize large intermediary DNA segments. One particular FB family member, FB-NOF, is associated with TE28, the white-crimson (w) mutant, the w-derived Tp w-1 and probably also with Tp w. A unique sequence located close to the white end of TE28 was used to clone the borders of TE77 and the surrounding sequences in the bithorax region, indicating that the TE can be used as a probe for gene isolation. Some evolutionary implications of the large composite transposons are discussed.

Citing Articles

Highly structured sequence homology between an insertion element and the gene in which it resides.

Rhodes P, Vodkin L Proc Natl Acad Sci U S A. 1985; 82(2):493-7.

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An FB-NOF mediated duplication of the white gene is responsible for the zeste1 phenotype in some Drosophila melanogaster unstable strains.

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FB elements can promote exon shuffling: a promoter-less white allele can be reactivated by FB mediated transposition in Drosophila melanogaster.

Moschetti R, Marsano R, Barsanti P, Caggese C, Caizzi R Mol Genet Genomics. 2004; 271(4):394-401.

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The instability of the TE-like mutation Dp(2:2)GYL of Drosophila melanogaster is intimately associated with the hobo element.

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References

Schedl P, Artavanis-Tsakonas S, Steward R, Gehring W, Mirault M, Goldschmidt-Clermont M . Two hybrid plasmids with D. melanogaster DNA sequences complementary to mRNA coding for the major heat shock protein. Cell. 1978; 14(4):921-9. DOI: 10.1016/0092-8674(78)90346-x. View

Potter S . DNA sequence analysis of a Drosophila foldback transposable element rearrangement. Mol Gen Genet. 1982; 188(1):107-10. DOI: 10.1007/BF00333002. View

Green M . Controlling element mediated transpositions of the white gene in Drosophila melanogaster. Genetics. 1969; 61(2):429-41. PMC: 1212168. DOI: 10.1093/genetics/61.2.429. View

Shiba T, Saigo K . Retrovirus-like particles containing RNA homologous to the transposable element copia in Drosophila melanogaster. Nature. 1983; 302(5904):119-24. DOI: 10.1038/302119a0. View

Fyrberg E, Davidson N, Kindle K . The actin genes of Drosophila: a dispersed multigene family. Cell. 1980; 19(2):365-78. DOI: 10.1016/0092-8674(80)90511-5. View

RASMUSON B, Montell I, Rasmuson A, Svahlin H, Westerberg B . Genetic instability in Drosophila melanogaster: evidence for regulation, excision and transposition at the white locus. Mol Gen Genet. 1980; 177(4):567-70. DOI: 10.1007/BF00272664. View

Ish-Horowicz D, Pinchin S . Genomic organization of the 87A7 and 87Cl heat-induced loci of Drosophila melanogaster. J Mol Biol. 1980; 142(2):231-45. DOI: 10.1016/0022-2836(80)90047-9. View

Rimm D, Horness D, Kucera J, Blattner F . Construction of coliphage lambda Charon vectors with BamHI cloning sites. Gene. 1980; 12(3-4):301-9. DOI: 10.1016/0378-1119(80)90113-4. View

Bingham P, Levis R, Rubin G . Cloning of DNA sequences from the white locus of D. melanogaster by a novel and general method. Cell. 1981; 25(3):693-704. DOI: 10.1016/0092-8674(81)90176-8. View

10.

Kleckner N . Transposable elements in prokaryotes. Annu Rev Genet. 1981; 15:341-404. DOI: 10.1146/annurev.ge.15.120181.002013. View

11.

Spradling A, Rubin G . Transposition of cloned P elements into Drosophila germ line chromosomes. Science. 1982; 218(4570):341-7. DOI: 10.1126/science.6289435. View

12.

Levis R, Collins M, Rubin G . FB elements are the common basis for the instability of the wDZL and wC Drosophila mutations. Cell. 1982; 30(2):551-65. DOI: 10.1016/0092-8674(82)90252-5. View

13.

Rubin G, Kidwell M, Bingham P . The molecular basis of P-M hybrid dysgenesis: the nature of induced mutations. Cell. 1982; 29(3):987-94. DOI: 10.1016/0092-8674(82)90462-7. View

14.

Goldberg M, Paro R, Gehring W . Molecular cloning of the white locus region of Drosophila melanogaster using a large transposable element. EMBO J. 1982; 1(1):93-8. PMC: 553001. DOI: 10.1002/j.1460-2075.1982.tb01130.x. View

15.

Gausz J, Gyurkovics H, Bencze G, Awad A, Holden J, Ish-Horowicz D . Genetic characterization of the region between 86F1,2 and 87B15 on chromosome 3 of Drosophila melanogaster. Genetics. 1981; 98(4):775-89. PMC: 1214474. DOI: 10.1093/genetics/98.4.775. View

16.

Spradling A, Rubin G . Drosophila genome organization: conserved and dynamic aspects. Annu Rev Genet. 1981; 15:219-64. DOI: 10.1146/annurev.ge.15.120181.001251. View

17.

Bingham P . The Regulation of White Locus Expression: A Dominant Mutant Allele at the White Locus of DROSOPHILA MELANOGASTER. Genetics. 1980; 95(2):341-53. PMC: 1214230. DOI: 10.1093/genetics/95.2.341. View

18.

Maniatis T, Hardison R, Lacy E, Lauer J, OConnell C, Quon D . The isolation of structural genes from libraries of eucaryotic DNA. Cell. 1978; 15(2):687-701. DOI: 10.1016/0092-8674(78)90036-3. View

19.

Green M . Mapping a Drosophila melanogaster "controlling element" by interallelic crossing over. Genetics. 1969; 61(2):423-8. PMC: 1212167. DOI: 10.1093/genetics/61.2.423. View

20.

Green M . The genetics of a mutable gene at the white locus of Drosophila melanogaster. Genetics. 1967; 56(3):467-82. PMC: 1211634. DOI: 10.1093/genetics/56.3.467. View