Detection of Bone Marrow Micrometastasis and Microcirculating Disease in Rhabdomyosarcoma by a Real-time RT-PCR Assay

Overview

Journal J Cancer Res Clin Oncol

Specialty Oncology

Date 2006 Jan 26

PMID 16435141

Citations 19

Authors

Soledad Gallego

Anna Llort

Josep Roma

Constantino Sabado

Luis Gros

Josep Sanchez de Toledo

Affiliations

Soon will be listed here.

Abstract

Purpose: To assess if molecular detection of minimal disseminated disease by real-time reverse transcription and polymerase chain reaction (RT-PCR) could contribute to a better treatment stratification in patients with rhabdomyosarcoma (RMS).

Methods: Relative quantification of the tumor-mRNA present in serial samples of bone marrow (BM) and peripheral blood (PB) from 16 patients with RMS (7 alveolar and 9 embryonal) was performed by a real-time RT-PCR assay. Expression of MyoD1 and acetylcholine receptor (AChR) was analyzed in all samples, along with PAX3/7-FKHR in samples from alveolar tumors.

Results: A good correlation was found between the expression of PAX3/7-FKHR and AChR, while MyoD1 was more sensitive but less specific. In this study, patients with positive PB at the end of treatment showed a poorer prognosis than patients with negative PB. Moreover, in this patient cohort, metastatic relapses were preceded by the detection of microcirculating disease in all cases.

Conclusion: The detection of minimal circulating and micrometastatic disease by real-time RT-PCR, based on the expression of multiple genes, yields highly reproducible results. Patients with positive PB after treatment show poorer survival than patients without microcirculating disease.

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