Genetic and Phenotypic Diversity of Quorum-sensing Systems in Clinical and Environmental Isolates of Vibrio Cholerae

Overview

Journal Infect Immun

Publisher American Society for Microbiology

Specialty Allergy & Immunology

Date 2006 Jan 24

PMID 16428762

Citations 93

Authors

Adam Joelsson

Zhi Liu

Jun Zhu

Affiliations

Soon will be listed here.

Abstract

Vibrio cholerae is the causative agent of cholera, a severe and devastating diarrheal disease. V. cholerae lives naturally in various aquatic habitats during interepidemic periods. Recent studies reveal that quorum-sensing systems, which exist in many bacteria and help them monitor their population densities and regulate various cellular functions, control V. cholerae pathogenesis, biofilm formation, and protease production. In this study we surveyed quorum-sensing systems in 16 geographically diverse V. cholerae strains from epidemic-causing O1 and O139 strains as well as non-O1/non-O139 and environmental isolates and discovered an unexpectedly high rate of dysfunctional components. We also found that a functional quorum-sensing system conferred a survival advantage on bacteria in biofilms when the bacteria were exposed to seawater, though quorum sensing was less important to survival in a planktonic state under the same conditions. These findings suggest that variations in quorum-sensing systems are due to environmental selective pressures and might be beneficial to V. cholerae's fitness under certain conditions found in its natural reservoirs.

Citing Articles

Identification of critical amino acids in the DNA binding domain of LuxO: Lessons from a constitutive active LuxO.

Surin S, Singh R, Kaur M, Choudhury G, Sen H, Dureja C PLoS One. 2024; 19(9):e0310444.

PMID: 39288109 PMC: 11407668. DOI: 10.1371/journal.pone.0310444.

Magnetic chitin beads (MCB) coated with reveals transcriptome dynamics in adult mice with a complex gut microbiota.

Qin Z, Chen G, Bao W, Ma Y, Yang X, Yi C Gut Microbes. 2023; 15(2):2274125.

PMID: 37934002 PMC: 10631443. DOI: 10.1080/19490976.2023.2274125.

Dps-dependent in vivo mutation enhances long-term host adaptation in Vibrio cholerae.

Luo M, Chen G, Yi C, Xue B, Yang X, Ma Y PLoS Pathog. 2023; 19(3):e1011250.

PMID: 36928244 PMC: 10104298. DOI: 10.1371/journal.ppat.1011250.

PAS Domain-Containing Chemoreceptors Influence the Signal Sensing and Intestinal Colonization of .

Shu R, Yuan C, Liu B, Song Y, Hou L, Ren P Genes (Basel). 2022; 13(12).

PMID: 36553491 PMC: 9777591. DOI: 10.3390/genes13122224.

Quorum-sensing- and type VI secretion-mediated spatiotemporal cell death drives genetic diversity in Vibrio cholerae.

Mashruwala A, Qin B, Bassler B Cell. 2022; 185(21):3966-3979.e13.

PMID: 36167071 PMC: 9623500. DOI: 10.1016/j.cell.2022.09.003.

References

Faruque A, Mahalanabis D, Islam A, Hoque S . Severity of cholera during concurrent infections with other enteric pathogens. J Diarrhoeal Dis Res. 1994; 12(3):214-8. View

Gardel C, Mekalanos J . Regulation of cholera toxin by temperature, pH, and osmolarity. Methods Enzymol. 1994; 235:517-26. DOI: 10.1016/0076-6879(94)35167-8. View

Sengupta D, Finkelstein R . Antibody against the capsule of Vibrio cholerae O139 protects against experimental challenge. Infect Immun. 1996; 64(1):343-5. PMC: 173765. DOI: 10.1128/iai.64.1.343-345.1996. View

Ehara M, Shimodori S, Kojima F, Ichinose Y, Hirayama T, Albert M . Characterization of filamentous phages of Vibrio cholerae O139 and O1. FEMS Microbiol Lett. 1997; 154(2):293-301. DOI: 10.1111/j.1574-6968.1997.tb12659.x. View

Jobling M, Holmes R . Characterization of hapR, a positive regulator of the Vibrio cholerae HA/protease gene hap, and its identification as a functional homologue of the Vibrio harveyi luxR gene. Mol Microbiol. 1998; 26(5):1023-34. DOI: 10.1046/j.1365-2958.1997.6402011.x. View

Surette M, Bassler B . Quorum sensing in Escherichia coli and Salmonella typhimurium. Proc Natl Acad Sci U S A. 1998; 95(12):7046-50. PMC: 22733. DOI: 10.1073/pnas.95.12.7046. View

Ikema M, Honma Y . A novel filamentous phage, fs-2, of Vibrio cholerae O139. Microbiology (Reading). 1998; 144 ( Pt 7):1901-1906. DOI: 10.1099/00221287-144-7-1901. View

Faruque S, Albert M, Mekalanos J . Epidemiology, genetics, and ecology of toxigenic Vibrio cholerae. Microbiol Mol Biol Rev. 1998; 62(4):1301-14. PMC: 98947. DOI: 10.1128/MMBR.62.4.1301-1314.1998. View

Skorupski K, Taylor R . A new level in the Vibrio cholerae ToxR virulence cascade: AphA is required for transcriptional activation of the tcpPH operon. Mol Microbiol. 1999; 31(3):763-71. DOI: 10.1046/j.1365-2958.1999.01215.x. View

10.

Costerton J, Stewart P, Greenberg E . Bacterial biofilms: a common cause of persistent infections. Science. 1999; 284(5418):1318-22. DOI: 10.1126/science.284.5418.1318. View

11.

Dziejman M, Serruto D, Tam V, Sturtevant D, Diraphat P, Faruque S . Genomic characterization of non-O1, non-O139 Vibrio cholerae reveals genes for a type III secretion system. Proc Natl Acad Sci U S A. 2005; 102(9):3465-70. PMC: 552950. DOI: 10.1073/pnas.0409918102. View

12.

Matz C, McDougald D, Moreno A, Yung P, Yildiz F, Kjelleberg S . Biofilm formation and phenotypic variation enhance predation-driven persistence of Vibrio cholerae. Proc Natl Acad Sci U S A. 2005; 102(46):16819-24. PMC: 1283802. DOI: 10.1073/pnas.0505350102. View

13.

Benitez J, Silva A, Finkelstein R . Environmental signals controlling production of hemagglutinin/protease in Vibrio cholerae. Infect Immun. 2001; 69(10):6549-53. PMC: 98796. DOI: 10.1128/IAI.69.10.6549-6553.2001. View

14.

Dziejman M, Balon E, Boyd D, Fraser C, Heidelberg J, Mekalanos J . Comparative genomic analysis of Vibrio cholerae: genes that correlate with cholera endemic and pandemic disease. Proc Natl Acad Sci U S A. 2002; 99(3):1556-61. PMC: 122229. DOI: 10.1073/pnas.042667999. View

15.

Zhu J, Miller M, Vance R, Dziejman M, Bassler B, Mekalanos J . Quorum-sensing regulators control virulence gene expression in Vibrio cholerae. Proc Natl Acad Sci U S A. 2002; 99(5):3129-34. PMC: 122484. DOI: 10.1073/pnas.052694299. View

16.

Faruque S, Nair G . Molecular ecology of toxigenic Vibrio cholerae. Microbiol Immunol. 2002; 46(2):59-66. DOI: 10.1111/j.1348-0421.2002.tb02659.x. View

17.

Reidl J, Klose K . Vibrio cholerae and cholera: out of the water and into the host. FEMS Microbiol Rev. 2002; 26(2):125-39. DOI: 10.1111/j.1574-6976.2002.tb00605.x. View

18.

Miller M, Skorupski K, Lenz D, Taylor R, Bassler B . Parallel quorum sensing systems converge to regulate virulence in Vibrio cholerae. Cell. 2002; 110(3):303-14. DOI: 10.1016/s0092-8674(02)00829-2. View

19.

Kovacikova G, Skorupski K . Regulation of virulence gene expression in Vibrio cholerae by quorum sensing: HapR functions at the aphA promoter. Mol Microbiol. 2002; 46(4):1135-47. DOI: 10.1046/j.1365-2958.2002.03229.x. View

20.

Faruque S, Sack D, Sack R, Colwell R, Takeda Y, Nair G . Emergence and evolution of Vibrio cholerae O139. Proc Natl Acad Sci U S A. 2003; 100(3):1304-9. PMC: 298768. DOI: 10.1073/pnas.0337468100. View