Salmonella Enterica Strains Lacking the Frataxin Homolog CyaY Show Defects in Fe-S Cluster Metabolism in Vivo

Overview

Journal J Bacteriol

Publisher American Society for Microbiology

Specialty Microbiology

Date 2006 Jan 24

PMID 16428423

Citations 26

Authors

E Vivas

E Skovran

D M Downs

Affiliations

Soon will be listed here.

Abstract

In Salmonella enterica, the isc operon contains genes necessary for the synthesis of Fe-S clusters and strains lacking this operon have severe defects in a variety of cellular processes. Other cellular loci that impact Fe-S cluster synthesis to a lesser extent have been described. The cyaY locus encodes a frataxin homolog, and it is shown here that lesions in this locus affect Fe-S cluster metabolism. When present in combination with other lesions, mutations in cyaY can result in a strain with more severe defects than those lacking the isc locus.

Citing Articles

An In Silico Analysis of Genetic Variants and Structural Modeling of the Human Frataxin Protein in Friedreich's Ataxia.

Da Conceicao L, Cabral L, Pereira G, De Mesquita J Int J Mol Sci. 2024; 25(11).

PMID: 38891993 PMC: 11172458. DOI: 10.3390/ijms25115796.

Repair of Iron Center Proteins-A Different Class of Hemerythrin-like Proteins.

Silva L, Matias P, Romao C, Saraiva L Molecules. 2022; 27(13).

PMID: 35807291 PMC: 9268430. DOI: 10.3390/molecules27134051.

The Multifaceted Bacterial Cysteine Desulfurases: From Metabolism to Pathogenesis.

Das M, Dewan A, Shee S, Singh A Antioxidants (Basel). 2021; 10(7).

PMID: 34201508 PMC: 8300815. DOI: 10.3390/antiox10070997.

How Is Fe-S Cluster Formation Regulated?.

Mettert E, Kiley P Annu Rev Microbiol. 2015; 69:505-26.

PMID: 26488283 PMC: 7487215. DOI: 10.1146/annurev-micro-091014-104457.

Trading Places-Switching Frataxin Function by a Single Amino Acid Substitution within the [Fe-S] Cluster Assembly Scaffold.

Dean D, Dos Santos P PLoS Genet. 2015; 11(5):e1005192.

PMID: 25996679 PMC: 4440749. DOI: 10.1371/journal.pgen.1005192.

References

Frazzon J, Dean D . Formation of iron-sulfur clusters in bacteria: an emerging field in bioinorganic chemistry. Curr Opin Chem Biol. 2003; 7(2):166-73. DOI: 10.1016/s1367-5931(03)00021-8. View

Claas K, Weber S, Downs D . Lesions in the nuo operon, encoding NADH dehydrogenase complex I, prevent PurF-independent thiamine synthesis and reduce flux through the oxidative pentose phosphate pathway in Salmonella enterica serovar typhimurium. J Bacteriol. 1999; 182(1):228-32. PMC: 94264. DOI: 10.1128/JB.182.1.228-232.2000. View

Gralnick J, Downs D . The YggX protein of Salmonella enterica is involved in Fe(II) trafficking and minimizes the DNA damage caused by hydroxyl radicals: residue CYS-7 is essential for YggX function. J Biol Chem. 2003; 278(23):20708-15. DOI: 10.1074/jbc.M301577200. View

Hinchliffe P, Sazanov L . Organization of iron-sulfur clusters in respiratory complex I. Science. 2005; 309(5735):771-4. DOI: 10.1126/science.1113988. View

Skovran E, Lauhon C, Downs D . Lack of YggX results in chronic oxidative stress and uncovers subtle defects in Fe-S cluster metabolism in Salmonella enterica. J Bacteriol. 2004; 186(22):7626-34. PMC: 524902. DOI: 10.1128/JB.186.22.7626-7634.2004. View

Skovran E, Downs D . Lack of the ApbC or ApbE protein results in a defect in Fe-S cluster metabolism in Salmonella enterica serovar Typhimurium. J Bacteriol. 2002; 185(1):98-106. PMC: 141979. DOI: 10.1128/JB.185.1.98-106.2003. View

Campuzano V, Montermini L, Lutz Y, Cova L, Hindelang C, Jiralerspong S . Frataxin is reduced in Friedreich ataxia patients and is associated with mitochondrial membranes. Hum Mol Genet. 1997; 6(11):1771-80. DOI: 10.1093/hmg/6.11.1771. View

Pandolfo M . The molecular basis of Friedreich ataxia. Adv Exp Med Biol. 2003; 516:99-118. DOI: 10.1007/978-1-4615-0117-6_5. View

Yoon T, Cowan J . Frataxin-mediated iron delivery to ferrochelatase in the final step of heme biosynthesis. J Biol Chem. 2004; 279(25):25943-6. DOI: 10.1074/jbc.C400107200. View

10.

Chamberlain S, Koenig M, Richter A, Palau F, Pandolfo M . Molecular analysis of the Friedreich's ataxia locus. Adv Neurol. 1993; 61:193-204. View

11.

Gralnick J, Webb E, Beck B, Downs D . Lesions in gshA (Encoding gamma-L-glutamyl-L-cysteine synthetase) prevent aerobic synthesis of thiamine in Salmonella enterica serovar typhimurium LT2. J Bacteriol. 2000; 182(18):5180-7. PMC: 94667. DOI: 10.1128/JB.182.18.5180-5187.2000. View

12.

Duby G, Foury F, Ramazzotti A, Herrmann J, Lutz T . A non-essential function for yeast frataxin in iron-sulfur cluster assembly. Hum Mol Genet. 2002; 11(21):2635-43. DOI: 10.1093/hmg/11.21.2635. View

13.

Friedrich T, Bottcher B . The gross structure of the respiratory complex I: a Lego System. Biochim Biophys Acta. 2004; 1608(1):1-9. DOI: 10.1016/j.bbabio.2003.10.002. View

14.

Flint D, Allen R . Ironminus signSulfur Proteins with Nonredox Functions. Chem Rev. 1996; 96(7):2315-2334. DOI: 10.1021/cr950041r. View

15.

Mansy S, Cowan J . Iron-sulfur cluster biosynthesis: toward an understanding of cellular machinery and molecular mechanism. Acc Chem Res. 2004; 37(9):719-25. DOI: 10.1021/ar0301781. View

16.

Babcock M, De Silva D, Oaks R, Jiralerspong S, Montermini L, Pandolfo M . Regulation of mitochondrial iron accumulation by Yfh1p, a putative homolog of frataxin. Science. 1997; 276(5319):1709-12. DOI: 10.1126/science.276.5319.1709. View

17.

Flint D . Escherichia coli contains a protein that is homologous in function and N-terminal sequence to the protein encoded by the nifS gene of Azotobacter vinelandii and that can participate in the synthesis of the Fe-S cluster of dihydroxy-acid dehydratase. J Biol Chem. 1996; 271(27):16068-74. View

18.

Skovran E, Downs D . Metabolic defects caused by mutations in the isc gene cluster in Salmonella enterica serovar typhimurium: implications for thiamine synthesis. J Bacteriol. 2000; 182(14):3896-903. PMC: 94571. DOI: 10.1128/JB.182.14.3896-3903.2000. View

19.

Li D, Ohshima K, Jiralerspong S, Bojanowski M, Pandolfo M . Knock-out of the cyaY gene in Escherichia coli does not affect cellular iron content and sensitivity to oxidants. FEBS Lett. 1999; 456(1):13-6. DOI: 10.1016/s0014-5793(99)00896-0. View

20.

Huynen M, Snel B, Bork P, Gibson T . The phylogenetic distribution of frataxin indicates a role in iron-sulfur cluster protein assembly. Hum Mol Genet. 2001; 10(21):2463-8. DOI: 10.1093/hmg/10.21.2463. View