Pathological Mechanisms of Alcohol-induced Hepatic Portal Hypertension in Early Stage Fibrosis Rat Model

Overview

Journal World J Gastroenterol

Publisher Baishideng Publishing Group

Specialty Gastroenterology

Date 2006 Jan 21

PMID 16425420

Citations 10

Authors

Jian Li

Jian-Zhao Niu

Ji-Feng Wang

Yu Li

Xiao-Hua Tao

Affiliations

Soon will be listed here.

Abstract

Aim: To study the role of hepatic sinusoidal capillarization and perisinusoidal fibrosis in rats with alcohol-induced portal hypertension and to discuss the pathological mechanisms of alcohol-induced hepatic portal hypertension.

Methods: Fifty SD rats were divided into control group (n=20) and model group (n=30). Alcoholic liver fibrosis rat model was induced by intragastric infusion of a mixture containing alcohol, corn oil and pyrazole (1 000:250:3). Fifteen rats in each group were killed at wk 16. The diameter and pressure of portal vein were measured. Plasma hyaluronic acid (HA), type IV collagen (CoIV) and laminin (LN) were determined by radioimmunoassay. Liver tissue was fixed in formalin (10%) and 6-mum thick sections were routinely stained with Mallory and Sirius Red. Liver tissue was treated with rabbit polyclonal antibody against LN and ColIV. Hepatic non-parenchymal cells were isolated, total protein was extracted and separated by SDS-PAGE. MMP-2 and TIMP-1 protein expression was estimated by Western blotting.

Results: The diameter (2.207+/-0.096 vs 1.528+/-0.054 mm, P<0.01) and pressure (11.014+/-0.395 vs 8.533+/-0.274 mmHg, P<0.01) of portal vein were significantly higher in model group than those in the control group. Plasma HA (129.97+/-16.10 vs 73.09+/-2.38 ng/mL, P<0.01), ColIV (210.49+/-4.36 vs 89.65+/-4.42 ng/mL, P<0.01) and LN (105.00+/-7.29 vs 55.70+/-4.32 ng/mL, P<0.01) were upregulated in model group. Abundant collagen deposited around the central vein of lobules, hepatic sinusoids and hepatocytes in model group. ColI and ColIII increased remarkably and perisinusoids were almost surrounded by ColIII. Immunohistochemical staining showed that ColIV protein level (0.130+/-0.007 vs 0.032+/-0.004, P<0.01) and LN protein level (0.152+/-0.005 vs 0.029+/-0.005, P<0.01) were up-regulated remarkably in model group. MMP-2 protein expression (2.306+/-1.089 vs 0.612+/-0.081, P<0.01) and TIMP-1 protein expression (3.015+/-1.364 vs 0.446+/-0.009, P<0.01) in freshly isolated hepatic non-parenchymal cells were up-regulated in model group and TIMP-1 protein expression was evidently higher than MMP-2 protein expression (2.669+/-0.170 vs 1.695+/-0.008, P<0.05).

Conclusion: Hepatic sinusoidal capillarization and peri-sinusoidal fibrosis are responsible for alcohol-induced portal hypertension in rats.

Citing Articles

Portal Hypertension in Alcohol-Associated Hepatitis.

McConnell M, Iwakiri Y Curr Hepatol Rep. 2023; 22(2):67-73.

PMID: 37214274 PMC: 10075503. DOI: 10.1007/s11901-023-00601-y.

Deficiency in Inactive Rhomboid Protein2 (iRhom2) Alleviates Alcoholic Liver Fibrosis by Suppressing Inflammation and Oxidative Stress.

Liu Y, Kuang Q, Dai X, Zhan M, Zhou L, Zhu L Int J Mol Sci. 2022; 23(14).

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Braverman E, Dennen C, Gold M, Bowirrat A, Gupta A, Baron D Int J Environ Res Public Health. 2022; 19(9).

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Roles of Extract in Increasing Serum Nitric Oxide Concentration and Vascular Dilatation of Ethanol-Induced Hypertensive Rats.

Solfaine R, Muniroh L, Sadarman , Apriza , Irawan A Prev Nutr Food Sci. 2021; 26(2):186-191.

PMID: 34316483 PMC: 8276709. DOI: 10.3746/pnf.2021.26.2.186.

Inhibition of liver fibrosis using vitamin A-coupled liposomes to deliver matrix metalloproteinase-2 siRNA in vitro.

Li Y, Liu F, Ding F, Chen P, Tang M Mol Med Rep. 2015; 12(3):3453-3461.

PMID: 26017616 PMC: 4526069. DOI: 10.3892/mmr.2015.3842.

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