ATRIP Associates with Replication Protein A-coated SsDNA Through Multiple Interactions

Overview

Journal Proc Natl Acad Sci U S A

Specialty Science

Date 2006 Jan 13

PMID 16407120

Citations 61

Authors

Yuka Namiki

Lee Zou

Affiliations

Soon will be listed here.

Abstract

The ATR (ATM- and rad3-related)-mediated checkpoint pathway has a crucial role in regulating the cellular responses to DNA damage and DNA-replication stress. ATRIP (ATR-interacting protein), the regulatory partner of ATR, binds directly to replication protein A (RPA)-coated ssDNA and enables the ATR-ATRIP complex to recognize this DNA damage-induced structure. Here, we show that ATRIP associates with RPA-ssDNA through multiple interactions. Two major RPA-ssDNA-interacting domains of ATRIP were mapped to the regions flanking the conserved coiled-coil domain. In contrast to a recent article, we found that ATRIP mutants lacking the N terminus retained the ability to bind to RPA-ssDNA, suggesting that the multiple interactions between ATRIP and RPA-ssDNA may function redundantly in the recruitment of ATR-ATRIP. Unexpectedly, one internal region of ATRIP exhibited affinity to ssDNA, suggesting that ATRIP may interact with ssDNA in the ATRIP-RPA-ssDNA complex. Also, the N terminus of ATRIP associated with RPA-ssDNA in two distinct ways, indicating a dynamic and regulated association between ATRIP and RPA-ssDNA.

Citing Articles

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Partial wrapping of single-stranded DNA by replication protein A and modulation through phosphorylation.

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Wrapping of single-stranded DNA by Replication Protein A and modulation through phosphorylation.

Chadda R, Kaushik V, Ahmad I, Deveryshetty J, Holehouse A, Sigurdsson S bioRxiv. 2024; .

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Mechanistic insights into DNA damage recognition and checkpoint control in plants.

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