Transformation of Actinidia Eriantha: a Potential Species for Functional Genomics Studies in Actinidia

Overview

Journal Plant Cell Rep

Publisher Springer

Date 2006 Jan 13

PMID 16404600

Citations 10

Authors

Tianchi Wang

Yidong Ran

Ross G Atkinson

Andrew P Gleave

Dan Cohen

Affiliations

Soon will be listed here.

Abstract

Protocols were developed for regeneration and Agrobacterium-mediated transformation of Actinidia eriantha Benth. A. eriantha has a number of features that make it a useful tool for functional genomics in Actinidia: the vines are relatively small and non-vigorous in nature, flowers form all over the vine including on lower axillary branches and the species flowers prolifically in greenhouse conditions. Flowering and fruiting of transgenic A. eriantha plants was obtained within 2 years of transformation in a containment greenhouse. GUS (beta-glucuronidase) activity indicating stable expression of the uidA gene was observed in leaf, stem, root, petal and fruit tissues. Molecular evidence for incorporation of transgenes into the A. eriantha genome was obtained by PCR and DNA gel blot analysis. Inheritance of transgenic phenotypes was demonstrated in seedling progeny. Functional genomic studies in kiwifruit have been initiated using transgenic A. eriantha plants.

Citing Articles

Stable plastid transformation in kiwifruit ().

Chen Q, Wu Y, Wang Y, Zhang J, Li S aBIOTECH. 2025; 6(1):72-80.

PMID: 40060181 PMC: 11889296. DOI: 10.1007/s42994-024-00186-0.

Agrobacterium rhizogenes-mediated marker-free transformation and gene editing system revealed that AeCBL3 mediates the formation of calcium oxalate crystal in kiwifruit.

Li P, Zhang Y, Liang J, Hu X, He Y, Miao T Mol Hortic. 2024; 4(1):1.

PMID: 38167546 PMC: 10759683. DOI: 10.1186/s43897-023-00077-w.

Telomere-to-telomere and haplotype-resolved genome of the kiwifruit Actinidia eriantha.

Wang Y, Dong M, Wu Y, Zhang F, Ren W, Lin Y Mol Hortic. 2023; 3(1):4.

PMID: 37789444 PMC: 10515003. DOI: 10.1186/s43897-023-00052-5.

Characterization and functional validation of β-carotene hydroxylase genes in .

Xia H, Zhou Y, Lin Z, Guo Y, Liu X, Wang T Hortic Res. 2022; 9:uhac063.

PMID: 35611182 PMC: 9123235. DOI: 10.1093/hr/uhac063.

Chromosome-scale genome assembly of kiwifruit Actinidia eriantha with single-molecule sequencing and chromatin interaction mapping.

Tang W, Sun X, Yue J, Tang X, Jiao C, Yang Y Gigascience. 2019; 8(4).

PMID: 30942870 PMC: 6446220. DOI: 10.1093/gigascience/giz027.

References

Yao J, Cohen D, Atkinson R, Richardson K, Morris B . Regeneration of transgenic plants from the commercial apple cultivar Royal Gala. Plant Cell Rep. 2013; 14(7):407-12. DOI: 10.1007/BF00234044. View

De Bondt A, Eggermont K, Druart P, De Vil M, Goderis I, Vanderleyden J . Agrobacterium-mediated transformation of apple (Malus x domestica Borkh.): an assessment of factors affecting gene transfer efficiency during early transformation steps. Plant Cell Rep. 2013; 13(10):587-93. DOI: 10.1007/BF00234517. View

Church G, Gilbert W . Genomic sequencing. Proc Natl Acad Sci U S A. 1984; 81(7):1991-5. PMC: 345422. DOI: 10.1073/pnas.81.7.1991. View

Clough S, Bent A . Floral dip: a simplified method for Agrobacterium-mediated transformation of Arabidopsis thaliana. Plant J. 1999; 16(6):735-43. DOI: 10.1046/j.1365-313x.1998.00343.x. View

Hood E, Helmer G, Fraley R, Chilton M . The hypervirulence of Agrobacterium tumefaciens A281 is encoded in a region of pTiBo542 outside of T-DNA. J Bacteriol. 1986; 168(3):1291-301. PMC: 213636. DOI: 10.1128/jb.168.3.1291-1301.1986. View

Uematsu C, Murase M, Ichikawa H, Imamura J . Agrobacterium-mediated transformation and regeneration of kiwi fruit. Plant Cell Rep. 2013; 10(6-7):286-90. DOI: 10.1007/BF00193143. View

Sciaky D, Montoya A, Chilton M . Fingerprints of Agrobacterium Ti plasmids. Plasmid. 1978; 1(2):238-53. DOI: 10.1016/0147-619x(78)90042-2. View

Bajaj S, Mohanty A . Recent advances in rice biotechnology--towards genetically superior transgenic rice. Plant Biotechnol J. 2006; 3(3):275-307. DOI: 10.1111/j.1467-7652.2005.00130.x. View

Ream L, Gordon M, Nester E . Multiple mutations in the T region of the Agrobacterium tumefaciens tumor-inducing plasmid. Proc Natl Acad Sci U S A. 1983; 80(6):1660-4. PMC: 393662. DOI: 10.1073/pnas.80.6.1660. View

10.

Lee H, Humann J, Pitrak J, Cuperus J, Dawn Parks T, Whistler C . Translation start sequences affect the efficiency of silencing of Agrobacterium tumefaciens T-DNA oncogenes. Plant Physiol. 2003; 133(3):966-77. PMC: 281594. DOI: 10.1104/pp.103.026534. View

11.

Park S, Morris J, Park J, Hirschi K, Smith R . Efficient and genotype-independent Agrobacterium--mediated tomato transformation. J Plant Physiol. 2003; 160(10):1253-7. DOI: 10.1078/0176-1617-01103. View

12.

Garfinkel D, Simpson R, Ream L, White F, Gordon M, Nester E . Genetic analysis of crown gall: fine structure map of the T-DNA by site-directed mutagenesis. Cell. 1981; 27(1 Pt 2):143-53. DOI: 10.1016/0092-8674(81)90368-8. View

13.

Janssen B, Gardner R . The use of transient GUS expression to develop an Agrobacterium-mediated gene transfer system for kiwifruit. Plant Cell Rep. 2013; 13(1):28-31. DOI: 10.1007/BF00232310. View