Identification of a Calcium-binding Protein As a Calcium-dependent Regulator of Brain Adenylate Cyclase

Overview

Journal Proc Natl Acad Sci U S A

Specialty Science

Date 1975 Jan 1

PMID 164029

Citations 92

Authors

C O Brostrom

Y C Huang

B M BRECKENRIDGE

D J Wolff

Affiliations

Soon will be listed here.

Abstract

An activating factor of adenylate cyclase (EC 4.6.1.1) HAS BEEN OBTAINED FROM DETERGENT-DISPERSED PREPARATIONS OF PORCINE CEREBRAL CORTEX BY COLUMN CHROMATOGRAPHY ON ECTEOLA-cellulose. The factor was identified by acrylamide gel electrophoresis and by enzyme activation studies as the Ca2+-binding protein that regulates the activity of a brain cyclic nucleotide phosphodiesterase. This Ca2+-binding protein confers a Ca2+-dependent activation upon the adenylate cyclase, which is reversed by the subsequent addition of egta in excess of the free Ca2+. It is proposed that this Ca2+-dependent regulator controls enzymatic activities responsible for the synthesis of adenosine 3':5'-monophosphate and for the hydrolysis of guanosine 3':5'-monophosphate.

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