Expression of the UbiE Gene of Geobacillus Stearothermophilus V in Escherichia Coli K-12 Mediates the Evolution of Selenium Compounds into the Headspace of Selenite- and Selenate-amended Cultures

Overview

Journal Appl Environ Microbiol

Specialties Environmental Health
Microbiology

Date 2006 Jan 5

PMID 16391146

Citations 5

Authors

J W Swearingen Jr

D E Fuentes

M A Araya

M F Plishker

C P Saavedra

T G Chasteen

C C Vasquez

Affiliations

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Abstract

The ubiE gene of Geobacillus stearothermophilus V, with its own promoter, was cloned and introduced into Escherichia coli. The cloned gene complemented the ubiE gene deficiency of E. coli AN70. In addition, the expression of this gene in E. coli JM109 resulted in the evolution of volatile selenium compounds when these cells were grown in selenite- or selenate-amended media. These compounds were dimethyl selenide and dimethyl diselenide.

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