Diverse Range of Small Peptides Associated with High-density Lipoprotein

Overview

Journal Biochem Biophys Res Commun

Publisher Elsevier

Specialty Biochemistry

Date 2006 Jan 3

PMID 16386709

Citations 27

Authors

Glen L Hortin

Rong-Fong Shen

Brian M Martin

Alan T Remaley

Affiliations

Soon will be listed here.

Abstract

High-density lipoproteins (HDL) were examined as potential carriers of small peptides in plasma. HDL purified by density gradient centrifugation was delipidated and fractionated by size-exclusion chromatography under denaturing conditions. By HPLC and mass spectrometry, more than 100 peptide components were found in the size range from 1000 to 5000 Da. By sequence analysis, peptides were identified as fragments of proteins such as apolipoproteins, fibrinogen, alpha1-proteinase inhibitor, and transthyretin. The results indicate that purified HDL bears a complex range of small peptides. It is unclear whether the peptides have any significant functional role as apolipopeptides, but they may represent a pathway for peptide delivery or scavenging and a significant reservoir of plasma peptides for diagnostic evaluation.

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References

Bichsel V, Liotta L, Petricoin 3rd E . Cancer proteomics: from biomarker discovery to signal pathway profiling. Cancer J. 2001; 7(1):69-78. View

Lowenthal M, Mehta A, Frogale K, Bandle R, Araujo R, Hood B . Analysis of albumin-associated peptides and proteins from ovarian cancer patients. Clin Chem. 2005; 51(10):1933-45. DOI: 10.1373/clinchem.2005.052944. View

Schrader M, Schulz-Knappe P . Peptidomics technologies for human body fluids. Trends Biotechnol. 2002; 19(10 Suppl):S55-60. DOI: 10.1016/S0167-7799(01)01800-5. View

Dennis M, Zhang M, Meng Y, Kadkhodayan M, Kirchhofer D, Combs D . Albumin binding as a general strategy for improving the pharmacokinetics of proteins. J Biol Chem. 2002; 277(38):35035-43. DOI: 10.1074/jbc.M205854200. View

Rai A, Zhang Z, Rosenzweig J, Shih I, Pham T, Fung E . Proteomic approaches to tumor marker discovery. Arch Pathol Lab Med. 2002; 126(12):1518-26. DOI: 10.5858/2002-126-1518-PATTMD. View

Mackness M, Mackness B, Durrington P . Paraoxonase and coronary heart disease. Atheroscler Suppl. 2003; 3(4):49-55. DOI: 10.1016/s1567-5688(02)00046-6. View

Beaumont N, Skinner V, Tan T, Ramesh B, Byrne D, MacColl G . Ghrelin can bind to a species of high density lipoprotein associated with paraoxonase. J Biol Chem. 2003; 278(11):8877-80. DOI: 10.1074/jbc.C200575200. View

Kaplan B, Cojocaru M, Unsworth E, Knecht A, Martin B . Search for peptidic "middle molecules" in uremic sera: isolation and chemical identification of fibrinogen fragments. J Chromatogr B Analyt Technol Biomed Life Sci. 2003; 796(1):141-53. DOI: 10.1016/j.jchromb.2003.08.008. View

Matthews K, Mueller-Ortiz S, Wetsel R . Carboxypeptidase N: a pleiotropic regulator of inflammation. Mol Immunol. 2003; 40(11):785-93. DOI: 10.1016/j.molimm.2003.10.002. View

10.

Mehta A, Ross S, Lowenthal M, Fusaro V, Fishman D, Petricoin 3rd E . Biomarker amplification by serum carrier protein binding. Dis Markers. 2004; 19(1):1-10. PMC: 3851653. DOI: 10.1155/2003/104879. View

11.

Hortin G, Meilinger B, Drake S . Size-selective extraction of peptides from urine for mass spectrometric analysis. Clin Chem. 2004; 50(6):1092-5. DOI: 10.1373/clinchem.2003.030742. View

12.

Pisitkun T, Shen R, Knepper M . Identification and proteomic profiling of exosomes in human urine. Proc Natl Acad Sci U S A. 2004; 101(36):13368-73. PMC: 516573. DOI: 10.1073/pnas.0403453101. View

13.

Brewer Jr H, Remaley A, Neufeld E, Basso F, Joyce C . Regulation of plasma high-density lipoprotein levels by the ABCA1 transporter and the emerging role of high-density lipoprotein in the treatment of cardiovascular disease. Arterioscler Thromb Vasc Biol. 2004; 24(10):1755-60. DOI: 10.1161/01.ATV.0000142804.27420.5b. View

14.

BREWER Jr H, Ronan R, Meng M, Bishop C . Isolation and characterization of apolipoproteins A-I, A-II, and A-IV. Methods Enzymol. 1986; 128:223-46. DOI: 10.1016/0076-6879(86)28070-2. View

15.

Gotto Jr A, Pownall H, HAVEL R . Introduction to the plasma lipoproteins. Methods Enzymol. 1986; 128:3-41. DOI: 10.1016/0076-6879(86)28061-1. View

16.

Golaz O, Hughes G, Frutiger S, Paquet N, Bairoch A, Pasquali C . Plasma and red blood cell protein maps: update 1993. Electrophoresis. 1993; 14(11):1223-31. DOI: 10.1002/elps.11501401183. View

17.

Schmidt H, Remaley A, Stonik J, Ronan R, Wellmann A, Thomas F . Carboxyl-terminal domain truncation alters apolipoprotein A-I in vivo catabolism. J Biol Chem. 1995; 270(10):5469-75. DOI: 10.1074/jbc.270.10.5469. View

18.

Broze Jr G . Tissue factor pathway inhibitor and the current concept of blood coagulation. Blood Coagul Fibrinolysis. 1995; 6 Suppl 1:S7-13. DOI: 10.1097/00001721-199506001-00002. View

19.

Ribaya-Mercado J, Ordovas J, Russell R . Effect of beta-carotene supplementation on the concentrations and distribution of carotenoids, vitamin E, vitamin A, and cholesterol in plasma lipoprotein and non-lipoprotein fractions in healthy older women. J Am Coll Nutr. 1995; 14(6):614-20. DOI: 10.1080/07315724.1995.10718550. View

20.

Goulinet S, Chapman M . Plasma LDL and HDL subspecies are heterogenous in particle content of tocopherols and oxygenated and hydrocarbon carotenoids. Relevance to oxidative resistance and atherogenesis. Arterioscler Thromb Vasc Biol. 1997; 17(4):786-96. DOI: 10.1161/01.atv.17.4.786. View