Double Knockouts of Phospholipases Dzeta1 and Dzeta2 in Arabidopsis Affect Root Elongation During Phosphate-limited Growth but Do Not Affect Root Hair Patterning

Overview

Journal Plant Physiol

Specialty Physiology

Date 2005 Dec 31

PMID 16384909

Citations 81

Authors

Maoyin Li

Chunbo Qin

Ruth Welti

Xuemin Wang

Affiliations

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Abstract

Root elongation and root hair formation are important in nutrient absorption. We found that two Arabidopsis (Arabidopsis thaliana) phospholipase Ds (PLDs), PLDzeta1 and PLDzeta2, were involved in root elongation during phosphate limitation. PLDzeta1 and PLDzeta2 are structurally different from the majority of plant PLDs by having phox and pleckstrin homology domains. Both PLDzetas were expressed more in roots than in other tissues. It was reported previously that inducible suppression or inducible overexpression of PLDzeta1 affected root hair patterning. However, gene knockouts of PLDzeta1, PLDzeta2, or the double knockout of PLDzeta1 and PLDzeta2 showed no effect on root hair formation. The expression of PLDzetas increased in response to phosphate limitation. The elongation of primary roots in PLDzeta1 and PLDzeta2 double knockout mutants was slower than that of wild type and single knockout mutants. The loss of PLDzeta2, but not PLDzeta1, led to a decreased accumulation of phosphatidic acid in roots under phosphate-limited conditions. These results indicate that PLDzeta1 and PLDzeta2 play a role in regulating root development in response to nutrient limitation.

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References

Schachtman , Reid , Ayling . Phosphorus Uptake by Plants: From Soil to Cell. Plant Physiol. 1998; 116(2):447-53. PMC: 1539172. DOI: 10.1104/pp.116.2.447. View

Wang X . Phospholipase D in hormonal and stress signaling. Curr Opin Plant Biol. 2002; 5(5):408-14. DOI: 10.1016/s1369-5266(02)00283-2. View

Qin C, Wang X . The Arabidopsis phospholipase D family. Characterization of a calcium-independent and phosphatidylcholine-selective PLD zeta 1 with distinct regulatory domains. Plant Physiol. 2002; 128(3):1057-68. PMC: 152217. DOI: 10.1104/pp.010928. View

Alonso J, Stepanova A, Leisse T, Kim C, Chen H, Shinn P . Genome-wide insertional mutagenesis of Arabidopsis thaliana. Science. 2003; 301(5633):653-7. DOI: 10.1126/science.1086391. View

Shin H, Shin H, Dewbre G, Harrison M . Phosphate transport in Arabidopsis: Pht1;1 and Pht1;4 play a major role in phosphate acquisition from both low- and high-phosphate environments. Plant J. 2004; 39(4):629-42. DOI: 10.1111/j.1365-313X.2004.02161.x. View

Meyers B, Lee D, Vu T, Tej S, Edberg S, Matvienko M . Arabidopsis MPSS. An online resource for quantitative expression analysis. Plant Physiol. 2004; 135(2):801-13. PMC: 514116. DOI: 10.1104/pp.104.039495. View

Wanjie S, Welti R, Moreau R, Chapman K . Identification and quantification of glycerolipids in cotton fibers: reconciliation with metabolic pathway predictions from DNA databases. Lipids. 2005; 40(8):773-85. DOI: 10.1007/s11745-005-1439-4. View

Williamson L, Ribrioux S, Fitter A, Leyser H . Phosphate availability regulates root system architecture in Arabidopsis. Plant Physiol. 2001; 126(2):875-82. PMC: 111176. DOI: 10.1104/pp.126.2.875. View

Li W, Li M, Zhang W, Welti R, Wang X . The plasma membrane-bound phospholipase Ddelta enhances freezing tolerance in Arabidopsis thaliana. Nat Biotechnol. 2004; 22(4):427-33. DOI: 10.1038/nbt949. View

10.

Di Cristina M, Sessa G, Dolan L, Linstead P, Baima S, Ruberti I . The Arabidopsis Athb-10 (GLABRA2) is an HD-Zip protein required for regulation of root hair development. Plant J. 1996; 10(3):393-402. DOI: 10.1046/j.1365-313x.1996.10030393.x. View

11.

Hartel H, Dormann P, Benning C . DGD1-independent biosynthesis of extraplastidic galactolipids after phosphate deprivation in Arabidopsis. Proc Natl Acad Sci U S A. 2000; 97(19):10649-54. PMC: 27079. DOI: 10.1073/pnas.180320497. View

12.

Lynch J . Root Architecture and Plant Productivity. Plant Physiol. 1995; 109(1):7-13. PMC: 157559. DOI: 10.1104/pp.109.1.7. View

13.

Zhang W, Yu L, Zhang Y, Wang X . Phospholipase D in the signaling networks of plant response to abscisic acid and reactive oxygen species. Biochim Biophys Acta. 2005; 1736(1):1-9. DOI: 10.1016/j.bbalip.2005.07.004. View

14.

Sang Y, Cui D, Wang X . Phospholipase D and phosphatidic acid-mediated generation of superoxide in Arabidopsis. Plant Physiol. 2001; 126(4):1449-58. PMC: 117145. DOI: 10.1104/pp.126.4.1449. View

15.

Ma Z, Baskin T, Brown K, Lynch J . Regulation of root elongation under phosphorus stress involves changes in ethylene responsiveness. Plant Physiol. 2003; 131(3):1381-90. PMC: 166897. DOI: 10.1104/pp.012161. View

16.

Zhang W, Qin C, Zhao J, Wang X . Phospholipase D alpha 1-derived phosphatidic acid interacts with ABI1 phosphatase 2C and regulates abscisic acid signaling. Proc Natl Acad Sci U S A. 2004; 101(25):9508-13. PMC: 439007. DOI: 10.1073/pnas.0402112101. View

17.

Stewart Jr C, Via L . A rapid CTAB DNA isolation technique useful for RAPD fingerprinting and other PCR applications. Biotechniques. 1993; 14(5):748-50. View

18.

Masucci J, Rerie W, FOREMAN D, Zhang M, Galway M, Marks M . The homeobox gene GLABRA2 is required for position-dependent cell differentiation in the root epidermis of Arabidopsis thaliana. Development. 1996; 122(4):1253-60. DOI: 10.1242/dev.122.4.1253. View

19.

Dolan L, Janmaat K, Willemsen V, Linstead P, Poethig S, Roberts K . Cellular organisation of the Arabidopsis thaliana root. Development. 1993; 119(1):71-84. DOI: 10.1242/dev.119.1.71. View

20.

Anthony R, Henriques R, Helfer A, Meszaros T, Rios G, Testerink C . A protein kinase target of a PDK1 signalling pathway is involved in root hair growth in Arabidopsis. EMBO J. 2004; 23(3):572-81. PMC: 1271803. DOI: 10.1038/sj.emboj.7600068. View