In Vitro Effects of Crotalus Durissus Terrificus and Bothrops Jararaca Venoms on Giardia Duodenalis Trophozoites

Overview

Journal Parasitol Res

Specialty Parasitology

Date 2005 Dec 27

PMID 16378220

Citations 5

Authors

Lina Shinohara

Stella Fellipe de Freitas

Reinaldo Jose da Silva

Semiramis Guimaraes

Affiliations

Soon will be listed here.

Abstract

Considering the snake venoms' pharmacological properties and chemotherapeutic potential as well as the need for new alternatives for Giardia infection treatment, the present study was carried out aiming to evaluate the in vitro effects of crude Crotalus durissus terrificus and Bothrops jararaca venoms on the growth and adherence of Giardia duodenalis trophozoites. Trophozoites (10(6)) were exposed to serial twofold dilutions of C. durissus terrificus and B. jararaca venoms that ranged from 3.125 to 200 microg/ml and from 5 to 320 microg/ml, respectively. The two venoms inhibited the growth of trophozoites, and the level of inhibition varied according to the assayed concentrations and incubation times. The highest reduction of parasite growth was observed with increasing concentrations of the venoms. No effect on parasite adherence was observed. Light microscope observations revealed changes of the pear-shape aspect of the cell and reduction of flagellar beating frequency in the great part of the trophozoites. This is the first attempt to evaluate the in vitro effects of snake venoms on Giardia trophozoites. The findings stress the need for more investigations and prompt us to perform further studies to identify active principles with antigiardial effect.

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References

McAllister T, Annett C, Cockwill C, Olson M, Wang Y, Cheeke P . Studies on the use of Yucca schidigera to control giardiosis. Vet Parasitol. 2001; 97(2):85-99. DOI: 10.1016/s0304-4017(01)00394-6. View

Thompson R . Giardiasis as a re-emerging infectious disease and its zoonotic potential. Int J Parasitol. 2000; 30(12-13):1259-67. DOI: 10.1016/s0020-7519(00)00127-2. View

De Meo M, Vanelle P, Bernadini E, Laget M, Maldonado J, Jentzer O . Evaluation of the mutagenic and genotoxic activities of 48 nitroimidazoles and related imidazole derivatives by the Ames test and the SOS chromotest. Environ Mol Mutagen. 1992; 19(2):167-81. DOI: 10.1002/em.2850190212. View

Sousa M, Poiares-da-Silva J . The cytotoxic effects of ciprofloxacin in Giardia lamblia trophozoites. Toxicol In Vitro. 2001; 15(4-5):297-301. DOI: 10.1016/s0887-2333(01)00026-1. View

Lemee V, Zaharia I, Nevez G, Rabodonirina M, Brasseur P, Ballet J . Metronidazole and albendazole susceptibility of 11 clinical isolates of Giardia duodenalis from France. J Antimicrob Chemother. 2000; 46(5):819-21. DOI: 10.1093/jac/46.5.819. View

Harris J, Plummer S, Turner M, Lloyd D . The microaerophilic flagellate Giardia intestinalis: Allium sativum (garlic) is an effective antigiardial. Microbiology (Reading). 2000; 146 Pt 12:3119-3127. DOI: 10.1099/00221287-146-12-3119. View

da Silva R, da Silva M, Vilela L, Fecchio D . Antitumor effect of Bothrops jararaca venom. Mediators Inflamm. 2002; 11(2):99-104. PMC: 1781646. DOI: 10.1080/09629350220131953. View

Hayashi M, Camargo A . The Bradykinin-potentiating peptides from venom gland and brain of Bothrops jararaca contain highly site specific inhibitors of the somatic angiotensin-converting enzyme. Toxicon. 2005; 45(8):1163-70. DOI: 10.1016/j.toxicon.2005.02.017. View

Keister D . Axenic culture of Giardia lamblia in TYI-S-33 medium supplemented with bile. Trans R Soc Trop Med Hyg. 1983; 77(4):487-8. DOI: 10.1016/0035-9203(83)90120-7. View

10.

Chin J, Sheinin D, Rauth A . Screening for the mutagenicity of nitro-group containing hypoxic cell radiosensitizers using Salmonella typhimurium strains TA 100 and TA98. Mutat Res. 1978; 58(1):1-10. View

11.

Tempone A, Andrade Jr H, Spencer P, Lourenco C, Rogero J, Nascimento N . Bothrops moojeni venom kills Leishmania spp. with hydrogen peroxide generated by its L-amino acid oxidase. Biochem Biophys Res Commun. 2001; 280(3):620-4. DOI: 10.1006/bbrc.2000.4175. View

12.

Stiles B, Sexton F, Weinstein S . Antibacterial effects of different snake venoms: purification and characterization of antibacterial proteins from Pseudechis australis (Australian king brown or mulga snake) venom. Toxicon. 1991; 29(9):1129-41. DOI: 10.1016/0041-0101(91)90210-i. View

13.

Zieler H, Keister D, DVORAK J, Ribeiro J . A snake venom phospholipase A(2) blocks malaria parasite development in the mosquito midgut by inhibiting ookinete association with the midgut surface. J Exp Biol. 2002; 204(Pt 23):4157-67. DOI: 10.1242/jeb.204.23.4157. View

14.

Freitas S, Shinohara L, Sforcin J, Guimaraes S . In vitro effects of propolis on Giardia duodenalis trophozoites. Phytomedicine. 2006; 13(3):170-5. DOI: 10.1016/j.phymed.2004.07.008. View

15.

Goncalves A, Soares M, De Souza W, DaMatta R, Alves E . Ultrastructural alterations and growth inhibition of Trypanosoma cruzi and Leishmania major induced by Bothrops jararaca venom. Parasitol Res. 2002; 88(7):598-602. DOI: 10.1007/s00436-002-0626-3. View

16.

Saguchi K, Hagiwara-Saguchi Y, Murayama N, Ohi H, Fujita Y, Camargo A . Molecular cloning of serine proteinases from Bothrops jararaca venom gland. Toxicon. 2005; 46(1):72-83. DOI: 10.1016/j.toxicon.2005.03.011. View

17.

Barat L, Bloland P . Drug resistance among malaria and other parasites. Infect Dis Clin North Am. 1998; 11(4):969-87. DOI: 10.1016/s0891-5520(05)70400-1. View

18.

Bendesky A, Menendez D, Ostrosky-Wegman P . Is metronidazole carcinogenic?. Mutat Res. 2002; 511(2):133-44. DOI: 10.1016/s1383-5742(02)00007-8. View

19.

Fernandez-Gomez R, Zerrouk H, Sebti F, Loyens M, Benslimane A, Ouaissi M . Growth inhibition of Trypanosoma cruzi and Leishmania donovani infantum by different snake venoms: preliminary identification of proteins from Cerastes cerastes venom which interact with the parasites. Toxicon. 1994; 32(8):875-82. DOI: 10.1016/0041-0101(94)90366-2. View

20.

Edlind T, Hang T, Chakraborty P . Activity of the anthelmintic benzimidazoles against Giardia lamblia in vitro. J Infect Dis. 1990; 162(6):1408-11. DOI: 10.1093/infdis/162.6.1408. View