Acute Effect of Fatty Acids on Metabolism and Mitochondrial Coupling in Skeletal Muscle

Overview

Journal Biochim Biophys Acta

Specialties Biochemistry
Biophysics

Date 2005 Dec 27

PMID 16375848

Citations 29

Authors

Sandro M Hirabara

Leonardo R Silveira

Luciane C Alberici

Carol V G Leandro

Rafael H Lambertucci

Gisele C Polimeno

Maria F Cury Boaventura

Joaquim Procopio

Anibal E Vercesi

Rui Curi

Affiliations

Soon will be listed here.

Abstract

Acute effects of free fatty acids (FFA) were investigated on: (1) glucose oxidation, and UCP-2 and -3 mRNA and protein levels in 1 h incubated rat soleus and extensor digitorium longus (EDL) muscles, (2) mitochondrial membrane potential in cultured skeletal muscle cells, (3) respiratory activity and transmembrane electrical potential in mitochondria isolated from rat skeletal muscle, and (4) oxygen consumption by anesthetized rats. Long-chain FFA increased both basal and insulin-stimulated glucose oxidation in incubated rat soleus and EDL muscles and reduced mitochondrial membrane potential in C2C12 myotubes and rat skeletal muscle cells. Caprylic, palmitic, oleic, and linoleic acid increased O(2) consumption and decreased electrical membrane potential in isolated mitochondria from rat skeletal muscles. FFA did not alter UCP-2 and -3 mRNA and protein levels in rat soleus and EDL muscles. Palmitic acid increased oxygen consumption by anesthetized rats. These results suggest that long-chain FFA acutely lead to mitochondrial uncoupling in skeletal muscle.

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