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Detection of Beta-Glucosidase Activity in Polyacrylamide Gels with Esculin As Substrate

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Date 1994 Dec 1
PMID 16349468
Citations 16
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Abstract

beta-Glucosidase can be located after nondenaturing polyacrylamide gel electrophoresis by incubating the gel with 0.1% esculin and 0.03% ferric chloride. The esculetin released from esculin by beta-glucosidase action reacts with ferric ion to produce a black band, corresponding to the beta-glucosidase, against the transparent background.

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