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An Immunological Assay for Detection and Enumeration of Thermophilic Biomining Microorganisms

Overview
Journal Appl Environ Microbiol
Specialties Environmental Health
Microbiology
Date 1994 Sep 1
PMID 16349398
Citations 7
Authors
A M Amaro
K B Hallberg
E B Lindstrom
C A Jerez
Affiliations
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Abstract

A specific, fast, and sensitive nonradioactive immunobinding assay for the detection and enumeration of the moderate thermophile Thiobacillus caldus and the thermophilic archaeon Sulfolobus acidocaldarius was developed. It employs enhanced chemiluminescence or peroxidase-conjugated immunoglobulins in a dot or slot blotting system and is very convenient for monitoring thermophilic bioleaching microorganisms in effluents from industrial bioleaching processes.

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