Genetic Transformation System for the Fungal Soybean Pathogen Cercospora Kikuchii

Overview

Journal Appl Environ Microbiol

Specialties Environmental Health
Microbiology

Date 1991 Oct 1

PMID 16348566

Citations 6

Authors

R G Upchurch

M Ehrenshaft

D C Walker

L A Sanders

Affiliations

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Abstract

An altered beta-tubulin gene that confers resistance to the fungicide benomyl was isolated from a genomic library of a UV-induced mutant of Cercospora kikuchii and used as a selectable marker for transformation. The level of benomyl resistance conferred to the transformants was at least 150-fold greater than the intrinsic resistance of the C. kikuchii recipient protoplasts. In the majority of cases, the tubulin fragment was integrated at the native beta-tubulin locus, apparently by gene replacement or gene conversion. The frequency of transformation ranged from 0.2 to 6 transformants per mug of DNA, depending on the recipient strain. Transformation with linearized plasmid resulted in a higher frequency, without changing the type of integration event. Transformants were phenotypically stable after eight consecutive transfers on medium without benomyl. This is the first report of a genetic transformation system for a Cercospora species.

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