Article: Isolation of a DNA Probe for Lactobacillus curvatus

A genomic library of Lactobacillus curvatus DSM 20019 was constructed in bacteriophage lambda gt11. A 1.2-kilobase DNA probe specific for L. curvatus was isolated from this library. When this probe was hybridized to DNA from Lactobacillus isolates from different sources classified by conventional techniques, differing degrees of hybridization were obtained. This could imply that these isolates may have been incorrectly classified.

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References

1.

Southern E . Detection of specific sequences among DNA fragments separated by gel electrophoresis. J Mol Biol. 1975; 98(3):503-17. DOI: 10.1016/s0022-2836(75)80083-0. View

2.

Rubin F, Kopecko D, Noon K, Baron L . Development of a DNA probe to detect Salmonella typhi. J Clin Microbiol. 1985; 22(4):600-5. PMC: 268475. DOI: 10.1128/jcm.22.4.600-605.1985. View

3.

Barker Jr R, Suebsaeng L, Rooney W, Alecrim G, Dourado H, Wirth D . Specific DNA probe for the diagnosis of Plasmodium falciparum malaria. Science. 1986; 231(4744):1434-6. DOI: 10.1126/science.3513309. View

4.

Kuritza A, Getty C, Shaughnessy P, Hesse R, Salyers A . DNA probes for identification of clinically important Bacteroides species. J Clin Microbiol. 1986; 23(2):343-9. PMC: 268639. DOI: 10.1128/jcm.23.2.343-349.1986. View

5.

Franzen L, Westin G, Shabo R, Aslund L, Perlmann H, Persson T . Analysis of clinical specimens by hybridisation with probe containing repetitive DNA from Plasmodium falciparum. A novel approach to malaria diagnosis. Lancet. 1984; 1(8376):525-8. DOI: 10.1016/s0140-6736(84)90929-2. View

6.

Wirth D, Pratt D . Rapid identification of Leishmania species by specific hybridization of kinetoplast DNA in cutaneous lesions. Proc Natl Acad Sci U S A. 1982; 79(22):6999-7003. PMC: 347262. DOI: 10.1073/pnas.79.22.6999. View

7.

Rigby P, Dieckmann M, Rhodes C, Berg P . Labeling deoxyribonucleic acid to high specific activity in vitro by nick translation with DNA polymerase I. J Mol Biol. 1977; 113(1):237-51. DOI: 10.1016/0022-2836(77)90052-3. View

8.

McPheat W, McNally T . Isolation of a repeated DNA sequence from Bordetella pertussis. J Gen Microbiol. 1987; 133(2):323-30. DOI: 10.1099/00221287-133-2-323. View

9.

Hastings J, Holzapfel W . Conventional taxonomy of lactobacilli surviving radurization of meat. J Appl Bacteriol. 1987; 62(3):209-16. DOI: 10.1111/j.1365-2672.1987.tb02400.x. View

10.

Jagow J, Hill W . Enumeration by DNA colony hybridization of virulent Yersinia enterocolitica colonies in artificially contaminated food. Appl Environ Microbiol. 1986; 51(2):441-3. PMC: 238891. DOI: 10.1128/aem.51.2.441-443.1986. View

11.

Shaw B, Harding C . A numerical taxonomic study of lactic acid bacteria from vacuum-packed beef, pork, lamb and bacon. J Appl Bacteriol. 1984; 56(1):25-40. DOI: 10.1111/j.1365-2672.1984.tb04693.x. View

Isolation of a DNA Probe for Lactobacillus Curvatus