Cloning of the Pachysolen Tannophilus Xylulokinase Gene by Complementation in Escherichia Coli

Overview

Journal Appl Environ Microbiol

Specialties Environmental Health
Microbiology

Date 1987 Dec 1

PMID 16347513

Citations 3

Authors

P E Stevis

J J Huang

N W Ho

Affiliations

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Abstract

The gene coding for xylulokinase has been isolated from the yeast Pachysolen tannophilus by complementation of Escherichia coli xylulokinase (xylB) mutants. Through subcloning, the gene has been localized at one end of a 3.2-kilobase EcoRI-PstI fragment. Expression of the cloned gene was insensitive to glucose inhibition. Furthermore, the cloned gene did not cross-hybridize with E. coli and Saccharomyces cerevisiae xylulokinase genes.

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Molecular cloning of XYL3 (D-xylulokinase) from Pichia stipitis and characterization of its physiological function.

Jin Y, Jones S, Shi N, Jeffries T Appl Environ Microbiol. 2002; 68(3):1232-9.

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