Calculation of K and V from Substrate Concentration Versus Time Plot

Overview

Journal Appl Environ Microbiol

Specialties Environmental Health
Microbiology

Date 1979 Oct 1

PMID 16345452

Citations 6

Authors

G H Counotte

R A Prins

Affiliations

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Abstract

A simple method for the calculation of kinetic parameters (K(m), V(max)) under conditions of changing substrate concentrations is presented. An application of the method to detect shifts in groups involved in the utilization of a substrate in a mixed microbial culture is given.

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References

WILKINSON G . Statistical estimations in enzyme kinetics. Biochem J. 1961; 80:324-32. PMC: 1244002. DOI: 10.1042/bj0800324. View