Cytoskeletal Alterations in Interphase Cells of the Green Alga Spirogyra Decimina in Response to Heavy Metals Exposure: I. The Effect of Cadmium

Overview

Journal Protoplasma

Publisher Springer

Specialty Biology

Date 2005 Dec 8

PMID 16333580

Citations 7

Authors

P Pribyl

V Cepak

V Zachleder

Affiliations

Soon will be listed here.

Abstract

The aim of the study was to elucidate the effect of cadmium ions on the arrangement of the actin and tubulin cytoskeleton, as well as the relationships between cytoskeletal changes and growth processes in the green filamentous alga Spirogyra decimina. Batch cultures of algae were carried out under defined conditions in the presence of various cadmium concentrations. In control cells, the cytoskeleton appeared to be a transversely oriented pattern of both microtubules and actin filaments of various thickness in the cell cortex; colocalization of cortical microtubules and actin filaments was apparent. Microtubules were very sensitive to the presence of cadmium ions. Depending on the cadmium concentration and the time of exposure, microtubules disintegrated into short rod-shaped fragments or they completely disappeared. A steep increase in cell width and a decrease in growth rate accompanied (and probably ensued) a very rapid disintegration of microtubules. Actin filaments were more stable because they were disturbed several hours later than microtubules at any cadmium concentration used. When cadmium ions were washed out, the actin cytoskeleton was rebuilt even in cells in which actin filaments were completely disintegrated at higher cadmium concentrations (40 or 100 microM). The much more sensitive microtubules were regenerated after treatment with lower cadmium concentrations (10 or 15 microM) only.

Citing Articles

Cadmium affects microtubule organization and post-translational modifications of tubulin in seedlings of soybean (Glycine max L.).

Gzyl J, Chmielowska-Bak J, Przymusinski R, Gwozdz E Front Plant Sci. 2015; 6:937.

PMID: 26594217 PMC: 4635210. DOI: 10.3389/fpls.2015.00937.

Pb-induced avoidance-like chloroplast movements in fronds of Lemna trisulca L.

Samardakiewicz S, Krzeszowiec-Jelen W, Bednarski W, Jankowski A, Suski S, Gabrys H PLoS One. 2015; 10(2):e0116757.

PMID: 25646776 PMC: 4315572. DOI: 10.1371/journal.pone.0116757.

The nitrate reductase inhibitor, tungsten, disrupts actin microfilaments in Zea mays L.

Adamakis I, Panteris E, Eleftheriou E Protoplasma. 2013; 251(3):567-74.

PMID: 24091895 DOI: 10.1007/s00709-013-0556-1.

Changes of metal-induced toxicity by H2O 2/NO modulators in Scenedesmus quadricauda (Chlorophyceae).

Stork F, Backor M, Klejdus B, Hedbavny J, Kovacik J Environ Sci Pollut Res Int. 2013; 20(8):5502-11.

PMID: 23436059 DOI: 10.1007/s11356-013-1541-0.

Cadmium toxicity: effects on cytoskeleton, vesicular trafficking and cell wall construction.

Wan L, Zhang H Plant Signal Behav. 2012; 7(3):345-8.

PMID: 22499203 PMC: 3443916. DOI: 10.4161/psb.18992.

References

Bandyopadhyay D, Chatterjee A, DATTA A . Effect of cadmium on purified hepatic flavokinase: involvement of reactive -SH group(s) in the inactivation of flavokinase by cadmium. Life Sci. 1997; 60(21):1891-903. DOI: 10.1016/s0024-3205(97)00151-3. View

Iwata K, Tazawa M, Itoh T . Turgor pressure regulation and the orientation of cortical microtubules in Spirogyra cells. Plant Cell Physiol. 2001; 42(6):594-8. DOI: 10.1093/pcp/pce073. View

Sivaguru , Baluska , Volkmann , Felle , Horst . Impacts of aluminum on the cytoskeleton of the maize root apex. short-term effects on the distal part of the transition zone . Plant Physiol. 1999; 119(3):1073-82. PMC: 32089. DOI: 10.1104/pp.119.3.1073. View

Schoenmakers T, Klaren P, Flik G, Lock R, Pang P, Bonga S . Actions of cadmium on basolateral plasma membrane proteins involved in calcium uptake by fish intestine. J Membr Biol. 1992; 127(3):161-72. DOI: 10.1007/BF00231504. View

Green P . Pathways of cellular morphogenesis. A diversity in Nitella. J Cell Biol. 1965; 27(2):343-63. PMC: 2106723. DOI: 10.1083/jcb.27.2.343. View

Bisova K, Hendrychova J, Cepak V, Zachleder V . Cell growth and division processes are differentially sensitive to cadmium in Scenedesmus quadricauda. Folia Microbiol (Praha). 2004; 48(6):805-16. DOI: 10.1007/BF02931518. View

Hogetsu T, Shibaoka H . The change of pattern in microfibril arrangement on the inner surface of the cell wall of Closterium acerosum during cell growth. Planta. 2014; 140(1):7-14. DOI: 10.1007/BF00389373. View

DalleDonne I, Milzani A, Colombo R . Actin assembly by cadmium ions. Biochim Biophys Acta. 1997; 1357(1):5-17. DOI: 10.1016/s0167-4889(97)00008-6. View

Powlin S, Keng P, Miller R . Toxicity of cadmium in human trophoblast cells (JAr choriocarcinoma): role of calmodulin and the calmodulin inhibitor, zaldaride maleate. Toxicol Appl Pharmacol. 1997; 144(2):225-34. DOI: 10.1006/taap.1997.8135. View

10.

Christie N, Costa M . In vitro assessment of the toxicity of metal compounds : IV. Disposition of metals in cells: Interactions with membranes, glutathione, metallothionein, and DNA. Biol Trace Elem Res. 2013; 6(2):139-58. DOI: 10.1007/BF02916931. View

11.

Li W, Zhao Y, Chou I . Alterations in cytoskeletal protein sulfhydryls and cellular glutathione in cultured cells exposed to cadmium and nickel ions. Toxicology. 1993; 77(1-2):65-79. DOI: 10.1016/0300-483x(93)90138-i. View

12.

Goto Y, Ueda K . Microfilament bundles of F-actin inSpirogyra observed by fluorescence microscopy. Planta. 2013; 173(4):442-6. DOI: 10.1007/BF00958955. View

13.

Gomez-Mendikute A, Cajaraville M . Comparative effects of cadmium, copper, paraquat and benzo[a]pyrene on the actin cytoskeleton and production of reactive oxygen species (ROS) in mussel haemocytes. Toxicol In Vitro. 2003; 17(5-6):539-46. DOI: 10.1016/s0887-2333(03)00093-6. View

14.

Blancaflor , Jones , Gilroy . Alterations in the cytoskeleton accompany aluminum-induced growth inhibition and morphological changes in primary roots of maize . Plant Physiol. 1998; 118(1):159-72. PMC: 34852. DOI: 10.1104/pp.118.1.159. View

15.

Wang Z, Templeton D . Cellular factors mediate cadmium-dependent actin depolymerization. Toxicol Appl Pharmacol. 1996; 139(1):115-21. DOI: 10.1006/taap.1996.0149. View

16.

Grolig F . Nuclear centering in Spirogyra: force integration by microfilaments along microtubules. Planta. 1998; 204(1):54-63. DOI: 10.1007/s004250050229. View

17.

Kinne-Saffran E, Hulseweh M, Pfaff C, Kinne R . Inhibition of Na,K-ATPase by cadmium: different mechanisms in different species. Toxicol Appl Pharmacol. 1993; 121(1):22-9. DOI: 10.1006/taap.1993.1124. View

18.

Perrino B, Chou I . Role of calmodulin in cadmium-induced microtubule disassembly. Cell Biol Int Rep. 1986; 10(7):565-73. DOI: 10.1016/0309-1651(86)90031-7. View

19.

Nick P, Bergfeld R, Schafer E, Schopfer P . Unilateral reorientation of microtubules at the outer epidermal wall during photo- and gravitropic curvature of maize coleoptiles and sunflower hypocotyls. Planta. 1990; 181(2):162-8. DOI: 10.1007/BF02411533. View

20.

Yoshida K, Inoue N, Sonobe S, Shimmen T . Involvement of microtubules in rhizoid differentiation of Spirogyra species. Protoplasma. 2003; 221(3-4):227-35. DOI: 10.1007/s00709-002-0078-8. View