Global Gene Expression Profiles Reveal Significant Nuclear Reprogramming by the Blastocyst Stage After Cloning

Overview

Journal Proc Natl Acad Sci U S A

Specialty Science

Date 2005 Nov 30

PMID 16314565

Citations 45

Authors

Sadie L Smith

Robin E Everts

X Cindy Tian

Fuliang Du

Li-Ying Sung

Sandra L Rodriguez-Zas

Byeong-Seon Jeong

Jean-Paul Renard

Harris A Lewin

Xiangzhong Yang

Affiliations

Soon will be listed here.

Abstract

Nuclear transfer (NT) has potential applications in agriculture and biomedicine, but the technology is hindered by low efficiency. Global gene expression analysis of clones is important for the comprehensive study of nuclear reprogramming. Here, we compared global gene expression profiles of individual bovine NT blastocysts with their somatic donor cells and fertilized control embryos using cDNA microarray technology. The NT embryos' gene expression profiles were drastically different from those of their donor cells and closely resembled those of the naturally fertilized embryos. Our findings demonstrate that the NT embryos have undergone significant nuclear reprogramming by the blastocyst stage; however, problems may occur during redifferentiation for tissue genesis and organogenesis, and small reprogramming errors may be magnified downstream in development.

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