Pathogen-responsive Expression of Glycosyltransferase Genes UGT73B3 and UGT73B5 is Necessary for Resistance to Pseudomonas Syringae Pv Tomato in Arabidopsis

Overview

Journal Plant Physiol

Specialty Physiology

Date 2005 Nov 25

PMID 16306146

Citations 89

Authors

Mathilde Langlois-Meurinne

Claire M M Gachon

Patrick Saindrenan

Affiliations

Soon will be listed here.

Abstract

The genome sequencing of Arabidopsis (Arabidopsis thaliana) has revealed that secondary metabolism plant glycosyltransferases (UGTs) are encoded by an unexpectedly large multigenic family of 120 members. Very little is known about their actual function in planta, in particular during plant pathogen interactions. Among them, members of the group D are of particular interest since they are related to UGTs involved in stress-inducible responses in other plant species. We provide here a detailed analysis of the expression profiles of this group of Arabidopsis UGTs following infection with Pseudomonas syringae pv tomato or after treatment with salicylic acid, methyljasmonate, and hydrogen peroxide. Members of the group D displayed distinct induction profiles, indicating potential roles in stress or defense responses notably for UGT73B3 and UGT73B5. Analysis of UGT expression in Arabidopsis defense-signaling mutants further revealed that their induction is methyljasmonate independent, but partially salicylic acid dependent. T-DNA tagged mutants (ugt73b3 and ugt73b5) exhibited decreased resistance to P. syringae pv tomato-AvrRpm1, indicating that expression of the corresponding UGT genes is necessary during the hypersensitive response. These results emphasize the importance of plant secondary metabolite UGTs in plant-pathogen interactions and provide foundation for future understanding of the exact role of UGTs during the hypersensitive response.

Citing Articles

Heterologous expression of Halostachys caspica pathogenesis-related protein 10 increases salt and drought resistance in transgenic Arabidopsis thaliana.

Cao J, Maitirouzi A, Feng Y, Zhang H, Heng Y, Zhang J Plant Mol Biol. 2024; 115(1):5.

PMID: 39671054 DOI: 10.1007/s11103-024-01536-8.

Genetic dissection of resistance to Phytophthora sojae using genome-wide association and linkage analysis in soybean [Glycine max (L.) Merr.].

You H, Jang I, Moon J, Kang I, Kim J, Kang S Theor Appl Genet. 2024; 137(12):263.

PMID: 39516394 DOI: 10.1007/s00122-024-04771-1.

Polychlorinated biphenyls modify root exudation pattern to accommodate degrading bacteria, showing strain and functional trait specificity.

Rolli E, Ghitti E, Mapelli F, Borin S Front Plant Sci. 2024; 15:1429096.

PMID: 39036359 PMC: 11258928. DOI: 10.3389/fpls.2024.1429096.

Transcriptomic Analysis for Diurnal Temperature Differences Reveals Gene-Regulation-Network Response to Accumulation of Bioactive Ingredients of Protocorm-like Bodies in .

Chen Q, Zhang C, Chen Y, Wang C, Lai Z Plants (Basel). 2024; 13(6).

PMID: 38592895 PMC: 10975105. DOI: 10.3390/plants13060874.

Genome-wide investigation of UDP-Glycosyltransferase family in Tartary buckwheat (Fagopyrum tataricum).

Yang F, Zhang L, Zhang X, Guan J, Wang B, Wu X BMC Plant Biol. 2024; 24(1):249.

PMID: 38580941 PMC: 10998406. DOI: 10.1186/s12870-024-04926-8.

References

Redman J, Haas B, Tanimoto G, Town C . Development and evaluation of an Arabidopsis whole genome Affymetrix probe array. Plant J. 2004; 38(3):545-61. DOI: 10.1111/j.1365-313X.2004.02061.x. View

Achnine L, Huhman D, Farag M, Sumner L, Blount J, Dixon R . Genomics-based selection and functional characterization of triterpene glycosyltransferases from the model legume Medicago truncatula. Plant J. 2005; 41(6):875-87. DOI: 10.1111/j.1365-313X.2005.02344.x. View

Edwards R, Dixon D, Walbot V . Plant glutathione S-transferases: enzymes with multiple functions in sickness and in health. Trends Plant Sci. 2000; 5(5):193-8. DOI: 10.1016/s1360-1385(00)01601-0. View

Lim E, Doucet C, Li Y, Elias L, Worrall D, Spencer S . The activity of Arabidopsis glycosyltransferases toward salicylic acid, 4-hydroxybenzoic acid, and other benzoates. J Biol Chem. 2001; 277(1):586-92. DOI: 10.1074/jbc.M109287200. View

Gachon C, Langlois-Meurinne M, Saindrenan P . Plant secondary metabolism glycosyltransferases: the emerging functional analysis. Trends Plant Sci. 2005; 10(11):542-9. DOI: 10.1016/j.tplants.2005.09.007. View

Alonso J, Stepanova A, Leisse T, Kim C, Chen H, Shinn P . Genome-wide insertional mutagenesis of Arabidopsis thaliana. Science. 2003; 301(5633):653-7. DOI: 10.1126/science.1086391. View

Gachon C, Baltz R, Saindrenan P . Over-expression of a scopoletin glucosyltransferase in Nicotiana tabacum leads to precocious lesion formation during the hypersensitive response to tobacco mosaic virus but does not affect virus resistance. Plant Mol Biol. 2004; 54(1):137-46. DOI: 10.1023/B:PLAN.0000028775.58537.fe. View

Asai T, Stone J, Heard J, Kovtun Y, Yorgey P, Sheen J . Fumonisin B1-induced cell death in arabidopsis protoplasts requires jasmonate-, ethylene-, and salicylate-dependent signaling pathways. Plant Cell. 2000; 12(10):1823-36. PMC: 149122. DOI: 10.1105/tpc.12.10.1823. View

Carpenter J, Kopczak S, Snustad D, Silflow C . Semi-constitutive expression of an Arabidopsis thaliana alpha-tubulin gene. Plant Mol Biol. 1993; 21(5):937-42. DOI: 10.1007/BF00027126. View

10.

Dooner H, Nelson O . Genetic control of UDPglucose:flavonol 3-O-glucosyltransferase in the endosperm of maize. Biochem Genet. 1977; 15(5-6):509-19. DOI: 10.1007/BF00520194. View

11.

Jones P, Messner B, Nakajima J, Schaffner A, Saito K . UGT73C6 and UGT78D1, glycosyltransferases involved in flavonol glycoside biosynthesis in Arabidopsis thaliana. J Biol Chem. 2003; 278(45):43910-8. DOI: 10.1074/jbc.M303523200. View

12.

Chong J, Baltz R, Schmitt C, Beffa R, Fritig B, Saindrenan P . Downregulation of a pathogen-responsive tobacco UDP-Glc:phenylpropanoid glucosyltransferase reduces scopoletin glucoside accumulation, enhances oxidative stress, and weakens virus resistance. Plant Cell. 2002; 14(5):1093-107. PMC: 150609. DOI: 10.1105/tpc.010436. View

13.

Li J, Ou-Lee T, Raba R, Amundson R, Last R . Arabidopsis Flavonoid Mutants Are Hypersensitive to UV-B Irradiation. Plant Cell. 1993; 5(2):171-179. PMC: 160260. DOI: 10.1105/tpc.5.2.171. View

14.

Moehs C, ALLEN P, Friedman M, Belknap W . Cloning and expression of solanidine UDP-glucose glucosyltransferase from potato. Plant J. 1997; 11(2):227-36. DOI: 10.1046/j.1365-313x.1997.11020227.x. View

15.

Vogt T, Jones P . Glycosyltransferases in plant natural product synthesis: characterization of a supergene family. Trends Plant Sci. 2000; 5(9):380-6. DOI: 10.1016/s1360-1385(00)01720-9. View

16.

Nishimura M, Stein M, Hou B, Vogel J, Edwards H, Somerville S . Loss of a callose synthase results in salicylic acid-dependent disease resistance. Science. 2003; 301(5635):969-72. DOI: 10.1126/science.1086716. View

17.

Fraissinet-Tachet L, Baltz R, Chong J, Kauffmann S, Fritig B, Saindrenan P . Two tobacco genes induced by infection, elicitor and salicylic acid encode glucosyltransferases acting on phenylpropanoids and benzoic acid derivatives, including salicylic acid. FEBS Lett. 1998; 437(3):319-23. DOI: 10.1016/s0014-5793(98)01257-5. View

18.

Kaminaga Y, Sahin F, Mizukami H . Molecular cloning and characterization of a glucosyltransferase catalyzing glucosylation of curcumin in cultured Catharanthus roseus cells. FEBS Lett. 2004; 567(2-3):197-202. DOI: 10.1016/j.febslet.2004.04.056. View

19.

Lieberherr D, Wagner U, Dubuis P, Metraux J, Mauch F . The rapid induction of glutathione S-transferases AtGSTF2 and AtGSTF6 by avirulent Pseudomonas syringae is the result of combined salicylic acid and ethylene signaling. Plant Cell Physiol. 2003; 44(7):750-7. DOI: 10.1093/pcp/pcg093. View

20.

Apel K, Hirt H . Reactive oxygen species: metabolism, oxidative stress, and signal transduction. Annu Rev Plant Biol. 2004; 55:373-99. DOI: 10.1146/annurev.arplant.55.031903.141701. View