QN1/KIAA1009: a New Essential Protein for Chromosome Segregation and Mitotic Spindle Assembly
Overview
Authors
Affiliations
We previously reported the involvement of QN1 (quail neuroretina 1) protein in cell cycle control during retinal development. We show here that QN1 is an ATPase conserved through evolution, from fugu to humans. We show that chicken/quail QN1 protein is orthologous to the KIAA1009 protein in humans, the function of which was not known. We demonstrate here for the first time that QN1/KIAA1009 protein is located at the spindle poles of the mitotic apparatus and at centrosomes during mitosis. The siRNA-mediated depletion of KIAA1009 led to abnormal mitosis with chromosome segregation defects and abnormal centrosome separation leading to the death of PC12 and MCF7 cells. Thus, QN1/KIAA1009 is a new microtubule-associated ATPase involved in cell division.
Nuzhat N, Van Schil K, Liakopoulos S, Bauwens M, Duenas Rey A, Kaseberg S J Clin Invest. 2023; 133(8).
PMID: 36862503 PMC: 10104899. DOI: 10.1172/JCI161156.
Genetic determinants of ammonia-induced acute lung injury in mice.
Bein K, Ganguly K, Martin T, Concel V, Brant K, Di Y Am J Physiol Lung Cell Mol Physiol. 2020; 320(1):L41-L62.
PMID: 33050709 PMC: 7847062. DOI: 10.1152/ajplung.00276.2020.
Genomewide association studies of suicide attempts in US soldiers.
Stein M, Ware E, Mitchell C, Chen C, Borja S, Cai T Am J Med Genet B Neuropsychiatr Genet. 2017; 174(8):786-797.
PMID: 28902444 PMC: 5685938. DOI: 10.1002/ajmg.b.32594.
Avidor-Reiss T, Ha A, Basiri M Cold Spring Harb Perspect Biol. 2017; 9(8).
PMID: 28108487 PMC: 5538411. DOI: 10.1101/cshperspect.a028142.
Oren M, Tarrant A, Alon S, Simon-Blecher N, Elbaz I, Appelbaum L Sci Rep. 2015; 5:11418.
PMID: 26081482 PMC: 4476465. DOI: 10.1038/srep11418.