Distribution of Heartwater in the Caribbean Determined on the Basis of Detection of Antibodies to the Conserved 32-kilodalton Protein of Cowdria Ruminantium

Overview

Journal J Clin Microbiol

Specialty Microbiology

Date 1992 Jul 1

PMID 1629346

Citations 7

Authors

A Muller Kubold

D Martinez

E Camus

F Jongejan

Affiliations

Soon will be listed here.

Abstract

A competitive enzyme-linked immunosorbent assay (cELISA) was developed to detect immunoglobulin G antibodies to the major 32-kDa protein of Cowdria ruminantium. A total of 1,804 serum samples collected from cattle on 19 islands in the eastern Caribbean Basin were tested by this cELISA. A total of 133 serum samples from 10 islands (Antigua, Dominica, Grenada, Guadeloupe, Martinique, Montserrat, St. Kitts, St. Lucia, St. Martin, and St. Vincent) were found to be positive. The presence of antibodies to C. ruminantium in cattle on these islands was confirmed by immunofluorescence and Western blotting (immunoblotting). In earlier studies, C. ruminantium has been demonstrated only on Guadeloupe, Antigua, and Marie Galante. This study shows that the causative agent of heartwater is now firmly established in the Caribbean.

Citing Articles

Detection by two enzyme-linked immunosorbent assays of antibodies to Ehrlichia ruminantium in field sera collected from sheep and cattle in Ghana.

Bell-Sakyi L, Koney E, Dogbey O, Sumption K, Walker A, Bath A Clin Diagn Lab Immunol. 2003; 10(5):917-25.

PMID: 12965927 PMC: 193896. DOI: 10.1128/cdli.10.5.917-925.2003.

Size variation of the major immunodominant protein of Cowdria ruminantium.

Barbet A, Semu S, Chigagure N, Kelly P, Jongejan F, Mahan S Clin Diagn Lab Immunol. 1994; 1(6):744-6.

PMID: 8556531 PMC: 368408. DOI: 10.1128/cdli.1.6.744-746.1994.

An immunoblotting diagnostic assay for heartwater based on the immunodominant 32-kilodalton protein of Cowdria ruminantium detects false positives in field sera.

Mahan S, Tebele N, Mukwedeya D, Semu S, Nyathi C, Wassink L J Clin Microbiol. 1993; 31(10):2729-37.

PMID: 8253974 PMC: 265996. DOI: 10.1128/jcm.31.10.2729-2737.1993.

Production of alpha interferon in Cowdria ruminantium-infected cattle and its effect on infected endothelial cell cultures.

Totte P, Jongejan F, de Gee A, Werenne J Infect Immun. 1994; 62(6):2600-4.

PMID: 8188383 PMC: 186550. DOI: 10.1128/iai.62.6.2600-2604.1994.

Correlation between antibodies to Cowdria ruminantium (rickettsiales) in cattle and the distribution of Amblyomma vector ticks in Zimbabwe.

de Vries N, Mahan S, Ushewokunze-Obatolu U, Norval R, Jongejan F Exp Appl Acarol. 1993; 17(11):799-810.

PMID: 7628226 DOI: 10.1007/BF00225853.

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