Alpha 2.0
Login Register
» Articles » PMID: 1629167

Anaerobic Control of Colicin E1 Production

Overview
Journal J Bacteriol
Publisher American Society for Microbiology
Specialty Microbiology
Date 1992 Aug 1
PMID 1629167
Citations 23
Authors
J M Eraso
G M Weinstock
Affiliations
Soon will be listed here.
Abstract

Expression of the cea gene, which is carried by the ColE1 plasmid and which encodes colicin E1, was found to be greatly increased when the cells were grown anaerobically. By using cea-lacZ fusions to quantitate expression, aerobic levels were found to be only a few percent of the anaerobic levels. The anaerobic increase in expression was observed both in protein and in operon fusions, indicating that its regulation occurred at the level of transcription. It was also found to require a functional fnr gene and to occur when the cea-lacZ fusion was present as a single copy in the bacterial chromosome instead of in the multicopy ColE1 plasmid. Anaerobic expression was regulated by the SOS response and catabolite repression as is aerobic expression. The start site of the mRNA produced under anaerobic conditions was mapped by primer extension and found to be the same as the start for mRNA produced under aerobic conditions. These observations show that the cea gene is anaerobically regulated and that the Fnr protein is a positive regulator of transcription of this gene.

Citing Articles

Colicin E2 expression in Escherichia coli biofilms: Induction and regulation revisited.

Bayramoglu-Guven B, Ghazaryan L, Toubiana D, Gillor O Curr Res Microb Sci. 2022; 3:100171.

PMID: 36518173 PMC: 9742991. DOI: 10.1016/j.crmicr.2022.100171.

A-Type Carrier Proteins Are Involved in [4Fe-4S] Cluster Insertion into the Radical -Adenosylmethionine Protein MoaA for the Synthesis of Active Molybdoenzymes.

Hasnat M, Zupok A, Olas J, Mueller-Roeber B, Leimkuhler S J Bacteriol. 2021; 203(12):e0008621.

PMID: 33782054 PMC: 8316096. DOI: 10.1128/JB.00086-21.

Fur-Dam Regulatory Interplay at an Internal Promoter of the Enteroaggregative Escherichia coli Type VI Secretion Gene Cluster.

Brunet Y, Bernard C, Cascales E J Bacteriol. 2020; 202(10).

PMID: 32152218 PMC: 7186456. DOI: 10.1128/JB.00075-20.

ChrASO, the chromate efflux pump of Shewanella oneidensis, improves chromate survival and reduction.

Baaziz H, Gambari C, Boyeldieu A, Ali Chaouche A, Alatou R, Mejean V PLoS One. 2017; 12(11):e0188516.

PMID: 29166414 PMC: 5699817. DOI: 10.1371/journal.pone.0188516.

The sulfur carrier protein TusA has a pleiotropic role in Escherichia coli that also affects molybdenum cofactor biosynthesis.

Dahl J, Radon C, Buhning M, Nimtz M, Leichert L, Denis Y J Biol Chem. 2013; 288(8):5426-42.

PMID: 23281480 PMC: 3581435. DOI: 10.1074/jbc.M112.431569.

References
1.
SANGER F, Nicklen S, Coulson A . DNA sequencing with chain-terminating inhibitors. Proc Natl Acad Sci U S A. 1977; 74(12):5463-7. PMC: 431765. DOI: 10.1073/pnas.74.12.5463. View
2.
Nakazawa A, Suzuki N, Tamada T . Requirements of glucose and incubation under static conditions for optimal colicin E1 induction. Antimicrob Agents Chemother. 1977; 11(2):219-24. PMC: 351956. DOI: 10.1128/AAC.11.2.219. View
3.
Nakazawa A, Tamada T . Stimulation of colicin E 1 synthesis by cyclic 3', 5'-adenosine monophosphate in mitomycin C-induced Escherichia coli. Biochem Biophys Res Commun. 1972; 46(2):1004-10. DOI: 10.1016/s0006-291x(72)80241-9. View
4.
Hull R . Effect of tsl mutations on Col E1 expression in a recA strain of Escherichia coli K-12. J Bacteriol. 1975; 123(2):775-6. PMC: 235792. DOI: 10.1128/jb.123.2.775-776.1975. View
5.
Casadaban M . Transposition and fusion of the lac genes to selected promoters in Escherichia coli using bacteriophage lambda and Mu. J Mol Biol. 1976; 104(3):541-55. DOI: 10.1016/0022-2836(76)90119-4. View