PSF1 is Essential for Early Embryogenesis in Mice

Overview

Journal Mol Cell Biol

Specialty Cell Biology

Date 2005 Nov 17

PMID 16287864

Citations 42

Authors

Masaya Ueno

Machiko Itoh

Lingyu Kong

Kazushi Sugihara

Masahide Asano

Nobuyuki Takakura

Affiliations

Soon will be listed here.

Abstract

Psf1 (partner of sld five 1) forms a novel heterotetramer complex, GINS (Go, Ichi, Nii, and San; five, one, two, and three, respectively, in Japanese), with Sld5, Psf2, and Psf3. The formation of this complex is essential for the initiation of DNA replication in yeast and Xenopus laevis egg extracts. Although all of the components are well conserved in higher eukaryotes, the biological function in vivo is largely unknown. We originally cloned the mouse ortholog of PSF1 from a hematopoietic stem cell cDNA library and found that PSF1 is expressed in blastocysts, adult bone marrow, and testis, in which the stem cell system is active. Here we used the gene-targeting technique to determine the physiological function of PSF1 in vivo. Mice homozygous for a nonfunctional mutant of PSF1 died in utero around the time of implantation. PSF1-/- blastocysts failed to show outgrowth in culture and exhibited a cell proliferation defect. Our data clearly indicate that PSF1 is required for early embryogenesis.

Citing Articles

[GINS1 Enhances Glycolysis, Proliferation and Metastasis in Lung Adenocarcinoma Cells by Activating the Notch/PI3K/AKT/mTORC1 Signaling Pathway].

Huo Y, Xu X, Ma X, Feng Y Zhongguo Fei Ai Za Zhi. 2024; 27(10):735-744.

PMID: 39631830 PMC: 11629005. DOI: 10.3779/j.issn.1009-3419.2024.101.27.

Comprehensive analysis of subunit expression, prognostic value, and immune infiltration in clear cell renal cell carcinoma.

Zhang Y, Sun Q, Meng W, Xie L, Li N, Zhang J Transl Androl Urol. 2024; 13(8):1517-1536.

PMID: 39280654 PMC: 11399050. DOI: 10.21037/tau-24-95.

OTU deubiquitinase, ubiquitin aldehyde binding 2 (OTUB2) modulates the stemness feature, chemoresistance, and epithelial-mesenchymal transition of colon cancer via regulating GINS complex subunit 1 (GINS1) expression.

Zhu W, Wu C, Liu Z, Zhao S, Huang J Cell Commun Signal. 2024; 22(1):420.

PMID: 39210373 PMC: 11361113. DOI: 10.1186/s12964-024-01789-2.

PAX5 and circ1857 affected DLBCL progression and B-cell proliferation through regulating GINS1.

Wang T, Chen Z, Li C, Zhang W, Huang W, Xue J Cancer Sci. 2023; 114(8):3203-3215.

PMID: 37221950 PMC: 10394140. DOI: 10.1111/cas.15856.

Comprehensive analysis of GINS subunits prognostic value and ceRNA network in sarcoma.

Zhou C, Chen Z, Xiao B, Xiang C, Li A, Zhao Z Front Cell Dev Biol. 2022; 10:951363.

PMID: 36092720 PMC: 9462653. DOI: 10.3389/fcell.2022.951363.

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