The Rapid Evaluation of Bacterial Growth in Blood Cultures by Selected Ion Flow Tube-mass Spectrometry (SIFT-MS) and Comparison with the BacT/ALERT Automated Blood Culture System

Overview

Journal J Microbiol Methods

Specialty Microbiology

Date 2005 Nov 8

PMID 16271408

Citations 24

Authors

J M Scotter

R A Allardyce

V S Langford

A Hill

D R Murdoch

Affiliations

Soon will be listed here.

Abstract

We compared the performance of the BacT/ALERT automated blood culture system with real-time, quantitative volatile organic compound (VOC) detection by selected ion flow tube-mass spectrometry (SIFT-MS). Blood samples from healthy donors were artificially infected with 5 or 100 CFU of organisms commonly causing bacteremia. Positive results by SIFT-MS analysis of headspace gases were recorded for 53/60 (88.3%) bottles at 8h, and 58/60 (96.6%) bottles at 24 h. We conclude that SIFT-MS is a sensitive method for the detection of microbial VOCs. Furthermore, profiles of the VOCs detected may allow simultaneous identification of infecting organisms.

Citing Articles

A matrix-centered view of mass spectrometry platform innovation for volatilome research.

Szeitz A, Sutton A, Hallam S Front Mol Biosci. 2024; 11:1421330.

PMID: 39539739 PMC: 11557394. DOI: 10.3389/fmolb.2024.1421330.

Progress and challenges of developing volatile metabolites from exhaled breath as a biomarker platform.

Chou H, Godbeer L, Allsworth M, Boyle B, Ball M Metabolomics. 2024; 20(4):72.

PMID: 38977623 PMC: 11230972. DOI: 10.1007/s11306-024-02142-x.

Blood Culture Headspace Gas Analysis Enables Early Detection of Bacteremia in an Animal Model of Sepsis.

Euler M, Perl T, Eickel I, Dudakova A, Maguilla Rosado E, Drees C Antibiotics (Basel). 2022; 11(8).

PMID: 35892382 PMC: 9331843. DOI: 10.3390/antibiotics11080992.

Recent Trends in Exhaled Breath Diagnosis Using an Artificial Olfactory System.

Kim C, Raja I, Lee J, Lee J, Kang M, Lee S Biosensors (Basel). 2021; 11(9).

PMID: 34562928 PMC: 8467588. DOI: 10.3390/bios11090337.

Virus-induced Volatile Organic Compounds Are Detectable in Exhaled Breath during Pulmonary Infection.

Kamal F, Kumar S, Edwards M, Veselkov K, Belluomo I, Kebadze T Am J Respir Crit Care Med. 2021; 204(9):1075-1085.

PMID: 34319857 PMC: 8663017. DOI: 10.1164/rccm.202103-0660OC.