A Gyrase Mutant with Low Activity Disrupts Supercoiling at the Replication Terminus

Overview

Journal J Bacteriol

Publisher American Society for Microbiology

Specialty Microbiology

Date 2005 Nov 4

PMID 16267301

Citations 10

Authors

Zhenhua Pang

Ray Chen

Dipankar Manna

N Patrick Higgins

Affiliations

Soon will be listed here.

Abstract

When a mutation in an essential gene shows a temperature-sensitive phenotype, one usually assumes that the protein is inactive at nonpermissive temperature. DNA gyrase is an essential bacterial enzyme composed of two subunits, GyrA and GyrB. The gyrB652 mutation results from a single base change that substitutes a serine residue for arginine 436 (R436-S) in the GyrB protein. At 42 degrees C, strains with the gyrB652 allele stop DNA replication, and at 37 degrees C, such strains grow but have RecA-dependent SOS induction and show constitutive RecBCD-dependent DNA degradation. Surprisingly, the GyrB652 protein is not inactive at 42 degrees C in vivo or in vitro and it doesn't directly produce breaks in chromosomal DNA. Rather, this mutant has a low k(cat) compared to wild-type GyrB subunit. With more than twice the normal mean number of supercoil domains, this gyrase hypomorph is prone to fork collapse and topological chaos near the terminus of DNA replication.

Citing Articles

Mechanisms of action of ribosomally synthesized and posttranslationally modified peptides (RiPPs).

Cao L, Do T, Link A J Ind Microbiol Biotechnol. 2021; 48(3-4).

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Supercoil Levels in and Chromosomes Are Regulated by the C-Terminal 35⁻38 Amino Acids of GyrA.

Rovinskiy N, Agbleke A, Chesnokova O, Higgins N Microorganisms. 2019; 7(3).

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Species-specific supercoil dynamics of the bacterial nucleoid.

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Topological Behavior of Plasmid DNA.

Higgins N, Vologodskii A Microbiol Spectr. 2015; 3(2).

PMID: 26104708 PMC: 4480603. DOI: 10.1128/microbiolspec.PLAS-0036-2014.

RNA polymerase: chromosome domain boundary maker and regulator of supercoil density.

Higgins N Curr Opin Microbiol. 2014; 22:138-43.

PMID: 25460807 PMC: 4359758. DOI: 10.1016/j.mib.2014.10.002.

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