Cross-reactivity of Antibody Against SARS-coronavirus Nucleocapsid Protein with IL-11

Overview

Journal Biochem Biophys Res Commun

Publisher Elsevier

Specialty Biochemistry

Date 2005 Nov 3

PMID 16263078

Citations 13

Authors

Man Cheng

Ceci W L Chan

Randy C F Cheung

Rama Kamesh Bikkavilli

Qi Zhao

Shannon W N Au

Paul K S Chan

Susanna S T Lee

Gregory Cheng

Walter K K Ho

Wing-Tai Cheung

Affiliations

Soon will be listed here.

Abstract

Infection of SARS-associated coronavirus (SARS-CoV) induced a strong anti-nucleocapsid (anti-N) antibody response. However, the pathophysiological significance of the anti-N antibodies in SARS pathogenesis is largely unknown. To profile the anti-N antibodies, a phage-displayed scFv library was prepared from mice immunized with heat-inactivated SARS-CoV-infected Vero E6 cell lysate. Specific anti-N scFvs were isolated by panning against a recombinant nucleocapsid protein and reactivity was confirmed with phage-ELISA. Sequence analysis indicated that two of the isolated anti-N scFv clones were identical and displayed a high homology with an scFv specific for interleukin 11 (IL-11), an anti-inflammatory cytokine derived from bone marrow stroma cells. In a neutralization assay, IL-11-induced STAT 3 phosphorylation in rat intestinal epithelial IEC-18 cells was completely suppressed by the anti-N scFv clone L9N01.

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